KsgA

• Description: rRNA adenine dimethyltransferase
  
  

Gene name	ksgA
Synonyms
Essential	no
Product	rRNA adenine dimethyltransferase
Function	rRNA maturation
Gene expression levels in SubtiExpress: ksgA
MW, pI	32 kDa, 5.712
Gene length, protein length	876 bp, 292 aa
Immediate neighbours	rnmV, yabG
Sequences	Protein DNA DNA_with_flanks
Genetic context This image was kindly provided by SubtiList
Expression at a glance   PubMed

Categories containing this gene/protein

translation, sporulation proteins

This gene is a member of the following regulons

SigF regulon

The gene

Basic information

• Locus tag: BSU00420

Database entries

• BsubCyc: BSU00420

• DBTBS entry: [1]

• SubtiList entry: [2]

The protein

Basic information/ Evolution

• Catalyzed reaction/ biological activity: methylation of A1518 and A1519 in 16S rRNA

• Protein family: KsgA subfamily (according to Swiss-Prot)

• Paralogous protein(s):

Extended information on the protein

• Kinetic information:

• Domains:

• Modification:

• Cofactor(s):

• Effectors of protein activity:

• Interactions:

• Localization:

Database entries

• BsubCyc: BSU00420

• Structure:

• UniProt: P37468

• KEGG entry: [3]

• E.C. number:

Expression and regulation

• Operon: rnmV-ksgA PubMed

• Expression browser: ksgA PubMed

• Sigma factor: SigF PubMed

• Regulation: expressed early during sporulation in the forespore (SigF) PubMed

• Regulatory mechanism:

• Additional information:

Biological materials

• Mutant:

• Expression vector:

• lacZ fusion:

• GFP fusion:

• two-hybrid system:

• Antibody:

References

Kozo Ochi, Ji-Yun Kim, Yukinori Tanaka, Guojun Wang, Kenta Masuda, Hideaki Nanamiya, Susumu Okamoto, Shinji Tokuyama, Yoshikazu Adachi, Fujio Kawamura
Inactivation of KsgA, a 16S rRNA methyltransferase, causes vigorous emergence of mutants with high-level kasugamycin resistance.
Antimicrob Agents Chemother: 2009, 53(1);193-201
[PubMed:19001112] [WorldCat.org] [DOI] (I p)

Stephanie T Wang, Barbara Setlow, Erin M Conlon, Jessica L Lyon, Daisuke Imamura, Tsutomu Sato, Peter Setlow, Richard Losick, Patrick Eichenberger
The forespore line of gene expression in Bacillus subtilis.
J Mol Biol: 2006, 358(1);16-37
[PubMed:16497325] [WorldCat.org] [DOI] (P p)

C Condon, D Brechemier-Baey, B Beltchev, M Grunberg-Manago, H Putzer
Identification of the gene encoding the 5S ribosomal RNA maturase in Bacillus subtilis: mature 5S rRNA is dispensable for ribosome function.
RNA: 2001, 7(2);242-53
[PubMed:11233981] [WorldCat.org] [DOI] (P p)