kinA

kinA
168

two-component sensor kinase, phosphorylates [protein|9896B99346B0D3F6D57F57377DB253B46135A37A|spo0F], part of the [wiki|phosphorelay]

locus
BSU_13990
Molecular weight
68.99 kDa
pI
5.49
Protein length
Gene length
function
initiation of sporulation
product
two-component sensor kinase
essential
no
synonyms
kinA, spoIIF, spoIIJ, gsiC, scoD

Genomic Context

Categories containing this gene/protein

List of homologs in different organisms, belongs to COG5805 (Galperin et al., 2021)

This gene is a member of the following regulons

Gene
Coordinates
1,470,026 → 1,471,846
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The protein
Catalyzed reaction/ biological activity
autophosphorylation, phosphorylation of [protein|9896B99346B0D3F6D57F57377DB253B46135A37A|spo0F]
mainly active in the older, inner regions of a colony (with [protein|2111AC1AE49D1006E10DC127BF5B7A0327DE94A7|kinB]) [Pubmed|21097618]
ATP + protein L-histidine --> ADP + protein N-phospho-L-histidine (according to UniProt)
[wiki|Domains]
three tandem [wiki|PAS domain]s in the N-terminal region of KinA, the [wiki|second PAS] domain is the major N-terminal determinant of KinA dimerization [Pubmed|23504013]
the first [wiki|PAS domain] is required for NAD(+) binding [Pubmed|23599347]
3 [wiki|PAS domain]s (aa 3-73, aa 140-214, aa 265-335) (according to UniProt)
2 [wiki|PAC domain]s (aa 77-116, aa 218-255) (according to UniProt)
[wiki|Histidine Kinase domain] (aa 402-606) (according to UniProt)
Structure
[PDB|2VLG] (PAS domain)
[AF|P16497]
Modification
autophosphorylation on a His residue
Effectors of protein activity
interaction with [protein|5A5E17D39296FECC1C03B1A7791AB1065BB4E4DF|pxpB] or [protein|CEFD10FAFA0DBC83CD2B61DAC9339FEE025B1611|sda] inhibits autophosphorylation of [protein|B29CAD04FB89ACC482EFCD29D50EDDA19145CAA2|kinA] [Pubmed|9334321,15023339]
autophosphorylation is inhibited by [protein|E5E2994D96FCECEE75E15136A08308C7B6C1C183|sivA] and [protein|E06A3392E644FEDC6F46ED41BBE89168F59B76B3|bslA] [Pubmed|23335417]
activated by interaction with NAD(+) that indicated poor respiratory activity of the cell [Pubmed|23599347], this has been falsified [pubmed|28449380]
[wiki|Localization]
cytoplasm
Expression and Regulation
Operons
genes
[gene|B29CAD04FB89ACC482EFCD29D50EDDA19145CAA2|kinA]
description
[Pubmed|1569009]
regulation
expressed under conditions that trigger sporulation ([wiki|Spo0A]) [Pubmed|9644251]
regulatory mechanism
[protein|2C54FE2ADC82FF414D732018C90649D477A925AD|spo0A]: activation, [Pubmed|9644251], in [regulon|protein:2C54FE2ADC82FF414D732018C90649D477A925AD|spo0A regulon]
stringent response: positive regulation, in [regulon|other_regulator:stringent response|stringent response]
sigma factors
[protein|DC3449D5F195E5C2E9E14FEC95396C8F1FDF73B4|sigH]: sigma factor, [Pubmed|1569009], in [regulon|protein:DC3449D5F195E5C2E9E14FEC95396C8F1FDF73B4|sigH regulon]
additional information
translation is repressed upon interaction of the mRNA with the [gene|FC93BA60BD185DE5EF31D5605F0B8294EC2E8E5A|sr1] RNA [pubmed|34478554]
promoter-up mutations or high artificial expression that render ''[wiki|kinA]'' independent from [wiki|SigH], allow [wiki|sporulation] at high salinity [Pubmed|26712348]
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[gene|B29CAD04FB89ACC482EFCD29D50EDDA19145CAA2|kinA]

2024-03-20 07:58:33

Jstuelk

66

0E62F7AD8CD3091271F54BC16A545F920A2475EE

E1D98A4CF39241213C2BB7A9EEEA407E06CC2045

Biological materials
Mutant
BKE13990 (Δ[gene|B29CAD04FB89ACC482EFCD29D50EDDA19145CAA2|kinA]::erm  trpC2) available at [http://bgsc.org/getdetail.php?bgscid=BKE13990 BGSC],  [Pubmed|28189581], upstream reverse: _UP1_CACAGAATCCCTCCTTTGCA,  downstream forward: _UP4_TTTCCAAAAAAATAAAAACA
BKK13990 (Δ[gene|B29CAD04FB89ACC482EFCD29D50EDDA19145CAA2|kinA]::kan  trpC2) available at [http://bgsc.org/getdetail.php?bgscid=BKK13990 BGSC],  [Pubmed|28189581], upstream reverse: _UP1_CACAGAATCCCTCCTTTGCA,  downstream forward: _UP4_TTTCCAAAAAAATAAAAACA
References
Reviews
22303284
Original Publications
32560401,23169620,20511506,19561131,20413551,1846779,11292798,16166384,11734624,9299348,12067336,18174125,10094672,11069677,15023339,2509430,17350039,9334321,9477965,9644251,1569009,20081035,21097618,21926229,22303282,22670053,23335417,23378509,23504013,23599347,25598361,26055117,26165942,26657919,26712348,27216630,28449380,18324779,28838935,34478554,35012345,36916921

B29CAD04FB89ACC482EFCD29D50EDDA19145CAA2

Page visits: 7908

Time of last update: 2024-03-28 12:38:43

Author of last update: Jstuelk