Difference between revisions of "KhtS"

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m (Jstuelk moved page YhaS to KhtS: 24330391)
(Biological materials)
 
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* '''Description:''' modulator of [[YhaU]] activity <br/><br/>
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* '''Description:''' modulator of [[KhtU]] activity <br/><br/>
 
{| align="right" border="1" cellpadding="2"  
 
{| align="right" border="1" cellpadding="2"  
 
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|style="background:#ABCDEF;" align="center"|'''Function''' || control of potassium ion efflux
 
|style="background:#ABCDEF;" align="center"|'''Function''' || control of potassium ion efflux
 
|-
 
|-
|colspan="2" style="background:#FAF8CC;" align="center"| '''Gene expression levels in [http://subtiwiki.uni-goettingen.de/apps/expression/ ''Subti''Express]''': [http://subtiwiki.uni-goettingen.de/apps/expression/expression.php?search=BSU09870 yhaS]
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|colspan="2" style="background:#FAF8CC;" align="center"| '''Gene expression levels in [http://subtiwiki.uni-goettingen.de/apps/expression/ ''Subti''Express]''': [http://subtiwiki.uni-goettingen.de/apps/expression/expression.php?search=BSU09870 khtS]
 +
|-
 +
|colspan="2" style="background:#FAF8CC;" align="center"| '''Metabolic function and regulation of this protein in [[SubtiPathways|''Subti''Pathways]]: <br/>[http://subtiwiki.uni-goettingen.de/subtipathways/search.php?enzyme=KhtS KhtS]'''
 
|-
 
|-
 
|style="background:#ABCDEF;" align="center"| '''MW, pI''' || 12 kDa, 7.023   
 
|style="background:#ABCDEF;" align="center"| '''MW, pI''' || 12 kDa, 7.023   
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|style="background:#ABCDEF;" align="center"| '''Gene length, protein length''' || 336 bp, 112 aa  
 
|style="background:#ABCDEF;" align="center"| '''Gene length, protein length''' || 336 bp, 112 aa  
 
|-
 
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|style="background:#ABCDEF;" align="center"|'''Immediate neighbours''' || ''[[yhaT]]'', ''[[yhaR]]''
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|style="background:#ABCDEF;" align="center"|'''Immediate neighbours''' || ''[[khtT]]'', ''[[yhaR]]''
 
|-
 
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|style="background:#FAF8CC;" align="center"|'''Sequences'''||[http://bsubcyc.org/BSUB/sequence-aa?type=GENE&object=BSU09870 Protein] [http://bsubcyc.org/BSUB/sequence?type=GENE&object=BSU09870 DNA] [http://bsubcyc.org/BSUB/seq-selector?chromosome=CHROM-1&object=BSU09870 DNA_with_flanks]
 
|style="background:#FAF8CC;" align="center"|'''Sequences'''||[http://bsubcyc.org/BSUB/sequence-aa?type=GENE&object=BSU09870 Protein] [http://bsubcyc.org/BSUB/sequence?type=GENE&object=BSU09870 DNA] [http://bsubcyc.org/BSUB/seq-selector?chromosome=CHROM-1&object=BSU09870 DNA_with_flanks]
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=Expression and regulation=
 
=Expression and regulation=
  
* '''Operon:''' ''[[yhaS]]-[[yhaT]]-[[yhaU]]'' {{PubMed|22383849}}
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* '''Operon:''' ''[[khtS]]-[[khtT]]-[[khtU]]'' {{PubMed|22383849}}
  
* '''Expression browser:''' [http://genome.jouy.inra.fr/cgi-bin/seb/viewdetail.py?id=khtS_1060988_1061326_-1 yhaS] {{PubMed|22383849}}
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* '''Expression browser:''' [http://genome.jouy.inra.fr/cgi-bin/seb/viewdetail.py?id=khtS_1060988_1061326_-1 khtS] {{PubMed|22383849}}
  
 
* '''[[Sigma factor]]:'''  
 
* '''[[Sigma factor]]:'''  
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* '''Mutant:'''
 
* '''Mutant:'''
 +
** GP2078 (''[[khtS]]-[[khtT]]-[[khtU]]''::''aphA3''), available in [[Jörg Stülke]]'s lab
  
 
* '''Expression vector:'''
 
* '''Expression vector:'''

Latest revision as of 08:29, 20 July 2015

  • Description: modulator of KhtU activity

Gene name yhaS
Synonyms
Essential no
Product modulator of YhaU activity
Function control of potassium ion efflux
Gene expression levels in SubtiExpress: khtS
Metabolic function and regulation of this protein in SubtiPathways:
KhtS
MW, pI 12 kDa, 7.023
Gene length, protein length 336 bp, 112 aa
Immediate neighbours khtT, yhaR
Sequences Protein DNA DNA_with_flanks
Genetic context
YhaS context.gif
This image was kindly provided by SubtiList
Expression at a glance   PubMed
YhaS expression.png















Categories containing this gene/protein

metal ion homeostasis (K, Na, Ca, Mg)

This gene is a member of the following regulons

The gene

Basic information

  • Locus tag: BSU09870

Phenotypes of a mutant

Database entries

  • DBTBS entry: no entry
  • SubtiList entry: [1]

Additional information

The protein

Basic information/ Evolution

  • Catalyzed reaction/ biological activity:
  • Protein family:
  • Paralogous protein(s):

Extended information on the protein

  • Kinetic information:
  • Modification:
  • Effectors of protein activity:

Database entries

  • Structure:
  • KEGG entry: [2]
  • E.C. number:

Additional information

Expression and regulation

  • Regulation:
  • Regulatory mechanism:
  • Additional information:

Biological materials

  • Expression vector:
  • lacZ fusion:
  • GFP fusion:
  • two-hybrid system:
  • Antibody:

Labs working on this gene/protein

Your additional remarks

References

Pete Chandrangsu, Renata Dusi, Chris J Hamilton, John D Helmann
Methylglyoxal resistance in Bacillus subtilis: contributions of bacillithiol-dependent and independent pathways.
Mol Microbiol: 2014, 91(4);706-15
[PubMed:24330391] [WorldCat.org] [DOI] (I p)

Makoto Fujisawa, Yuko Wada, Masahiro Ito
Modulation of the K+ efflux activity of Bacillus subtilis YhaU by YhaT and the C-terminal region of YhaS.
FEMS Microbiol Lett: 2004, 231(2);211-7
[PubMed:14987767] [WorldCat.org] [DOI] (P p)