kbl

168

2-amino-3-ketobutyrate CoA ligase

locus: BSU_17000

Molecular weight: 43.12 kDa

pI: 6.25

Protein length: 392 aa

Gene length: 1179 bp

function: threonine utilization

product: 2-amino-3-ketobutyrate CoA ligase

essential: no

ec: 2.3.1.29

synonyms: kbl

List of homologs in different organisms, belongs to COG0156 (Galperin et al., 2021)

Gene

Coordinates: 1,771,517  1,772,695

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The protein

Catalyzed reaction/ biological activity

• H⁺ + L-alanine + pimeloyl-[ACP] --> 8-amino-7-oxononanoate + CO₂ + holo-[ACP] (according to UniProt)

Protein family

• [wiki|class-II pyridoxal-phosphate-dependent aminotransferase family] (according to UniProt)

[wiki|Cofactors]

• PLP (according to UniProt)

Structure

• [PDB|1FC4] (from ''Escherichia coli'', 40% identity, 60% similarity) [Pubmed|11318637]
• [AF|O31777]

Paralogous protein(s)

• [protein|8B6A3E86A88928526D94FDA94B9C81ABF6E9ACFB|bioF]

Biological materials

Mutant

• BKE17000 ([gene|1BC05699DEB3A16944FB8305F22976AED75805E8|kbl]::erm  trpC2) available at [http://bgsc.org/getdetail.php?bgscid=BKE17000 BGSC],  [Pubmed|28189581], upstream reverse: _UP1_CATGTTTATCCCCCTTTATG,  downstream forward: _UP4_TAACAAGAGGAAGAGTTTAC
• BKK17000 ([gene|1BC05699DEB3A16944FB8305F22976AED75805E8|kbl]::kan  trpC2) available at [http://bgsc.org/getdetail.php?bgscid=BKK17000 BGSC],  [Pubmed|28189581], upstream reverse: _UP1_CATGTTTATCCCCCTTTATG,  downstream forward: _UP4_TAACAAGAGGAAGAGTTTAC

References

• 11318637,22960854