Difference between revisions of "IspH"

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|style="background:#ABCDEF;" align="center"|'''Immediate neighbours''' || ''[[yqfQ]]'', ''[[yqfO]]''
 
|style="background:#ABCDEF;" align="center"|'''Immediate neighbours''' || ''[[yqfQ]]'', ''[[yqfO]]''
 
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|style="background:#FAF8CC;" align="center"|'''Sequences'''||[http://bsubcyc.org/BSUB/sequence-aa?type=GENE&object=BSU25160 Protein] [http://bsubcyc.org/BSUB/sequence?type=GENE&object=BSU25160 DNA] [http://bsubcyc.org/BSUB/seq-selector?chromosome=CHROM-1&object=BSU25160 Advanced_DNA]
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|style="background:#FAF8CC;" align="center"|'''Sequences'''||[http://bsubcyc.org/BSUB/sequence-aa?type=GENE&object=BSU25160 Protein] [http://bsubcyc.org/BSUB/sequence?type=GENE&object=BSU25160 DNA] [http://bsubcyc.org/BSUB/seq-selector?chromosome=CHROM-1&object=BSU25160 DNA_with_flanks]
 
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Revision as of 10:44, 14 May 2013

  • Description: similar to penicillin tolerance, isopentenyl diphosphate biosynthesis

Gene name ispH
Synonyms yqfP
Essential yes PubMed
Product unknown
Function unknown
Gene expression levels in SubtiExpress: ispH
MW, pI 34 kDa, 5.615
Gene length, protein length 942 bp, 314 aa
Immediate neighbours yqfQ, yqfO
Sequences Protein DNA DNA_with_flanks
Genetic context
YqfP context.gif
This image was kindly provided by SubtiList
Expression at a glance   PubMed
IspH expression.png


























Categories containing this gene/protein

biosynthesis of lipids, essential genes

This gene is a member of the following regulons

The gene

Basic information

  • Locus tag: BSU25160

Phenotypes of a mutant

essential PubMed

Database entries

  • DBTBS entry: no entry
  • SubtiList entry: [1]

Additional information

The protein

Basic information/ Evolution

  • Catalyzed reaction/ biological activity: Isopentenyl diphosphate + NAD(P)+ + H2O = (E)-4-hydroxy-3-methylbut-2-en-1-yl diphosphate + NAD(P)H (according to Swiss-Prot)
  • Protein family: ispH family (according to Swiss-Prot)
  • Paralogous protein(s):

Extended information on the protein

  • Kinetic information:
  • Domains:
  • Modification:
  • Cofactor(s):
  • Effectors of protein activity:

Database entries

  • Structure:
  • KEGG entry: [2]
  • E.C. number:

Additional information

Expression and regulation

  • Operon:
  • Sigma factor:
  • Regulation:
  • Regulatory mechanism:
  • Additional information:

Biological materials

  • Mutant:
  • Expression vector:
  • lacZ fusion:
  • GFP fusion:
  • two-hybrid system:
  • Antibody:

Labs working on this gene/protein

Your additional remarks

References

Mattijs K Julsing, Michael Rijpkema, Herman J Woerdenbag, Wim J Quax, Oliver Kayser
Functional analysis of genes involved in the biosynthesis of isoprene in Bacillus subtilis.
Appl Microbiol Biotechnol: 2007, 75(6);1377-84
[PubMed:17458547] [WorldCat.org] [DOI] (P p)