Difference between revisions of "IspG"
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| − | * '''Description:''' similar to peptidoglycan acetylation, 1-hydroxy-2-methyl-2-(E)-butenyl-4-diphosphate synthase <br/><br/> | + | * '''Description:''' similar to peptidoglycan acetylation, 1-hydroxy-2-methyl-2-(E)-butenyl-4-diphosphate synthase, 6th step in the MEP pathway of isoprenoid biosynthesis <br/><br/> |
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|style="background:#ABCDEF;" align="center"| '''Essential''' || yes [http://www.ncbi.nlm.nih.gov/pubmed/12682299 PubMed] | |style="background:#ABCDEF;" align="center"| '''Essential''' || yes [http://www.ncbi.nlm.nih.gov/pubmed/12682299 PubMed] | ||
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| − | |style="background:#ABCDEF;" align="center"| '''Product''' || | + | |style="background:#ABCDEF;" align="center"| '''Product''' || 1-hydroxy-2-methyl-2-(E)-butenyl-4-diphosphate synthase |
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| − | |style="background:#ABCDEF;" align="center"|'''Function''' || | + | |style="background:#ABCDEF;" align="center"|'''Function''' || MEP pathway of isoprenoid biosynthesis |
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|colspan="2" style="background:#FAF8CC;" align="center"| '''Gene expression levels in [http://subtiwiki.uni-goettingen.de/apps/expression/ ''Subti''Express]''': [http://subtiwiki.uni-goettingen.de/apps/expression/expression.php?search=BSU25070 ispG] | |colspan="2" style="background:#FAF8CC;" align="center"| '''Gene expression levels in [http://subtiwiki.uni-goettingen.de/apps/expression/ ''Subti''Express]''': [http://subtiwiki.uni-goettingen.de/apps/expression/expression.php?search=BSU25070 ispG] | ||
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* '''Expression browser:''' [http://genome.jouy.inra.fr/cgi-bin/seb/viewdetail.py?id=ispG_2589123_2590256_1 ispG] {{PubMed|22383849}} | * '''Expression browser:''' [http://genome.jouy.inra.fr/cgi-bin/seb/viewdetail.py?id=ispG_2589123_2590256_1 ispG] {{PubMed|22383849}} | ||
| − | * '''Sigma factor:''' [[SigE]] {{PubMed|12662922}} | + | * '''[[Sigma factor]]:''' [[SigE]] {{PubMed|12662922}} |
* '''Regulation:''' | * '''Regulation:''' | ||
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=References= | =References= | ||
| − | <pubmed>12662922 15383836 17458547 </pubmed> | + | <pubmed>12662922 15383836 17458547 23832336 </pubmed> |
[[Category:Protein-coding genes]] | [[Category:Protein-coding genes]] | ||
Revision as of 08:11, 9 July 2013
- Description: similar to peptidoglycan acetylation, 1-hydroxy-2-methyl-2-(E)-butenyl-4-diphosphate synthase, 6th step in the MEP pathway of isoprenoid biosynthesis
| Gene name | ispG |
| Synonyms | yqfY |
| Essential | yes PubMed |
| Product | 1-hydroxy-2-methyl-2-(E)-butenyl-4-diphosphate synthase |
| Function | MEP pathway of isoprenoid biosynthesis |
| Gene expression levels in SubtiExpress: ispG | |
| MW, pI | 40 kDa, 6.802 |
| Gene length, protein length | 1131 bp, 377 aa |
| Immediate neighbours | yqfZ, yqfX |
| Sequences | Protein DNA DNA_with_flanks |
Genetic context 
This image was kindly provided by SubtiList
| |
Expression at a glance PubMed
| |
Contents
Categories containing this gene/protein
biosynthesis of lipids, sporulation proteins, essential genes
This gene is a member of the following regulons
The gene
Basic information
- Locus tag: BSU25070
Phenotypes of a mutant
essential PubMed
Database entries
- DBTBS entry: [1]
- SubtiList entry: [2]
Additional information
The protein
Basic information/ Evolution
- Catalyzed reaction/ biological activity: (E)-4-hydroxy-3-methylbut-2-en-1-yl diphosphate + H2O + protein-disulfide = 2-C-methyl-D-erythritol 2,4-cyclodiphosphate + protein-dithiol (according to Swiss-Prot)
- Protein family: ispG family (according to Swiss-Prot)
- Paralogous protein(s):
Extended information on the protein
- Kinetic information:
- Domains:
- Modification:
- Cofactor(s): contains an iron-sulfur cluster
- Effectors of protein activity:
- Localization:
- secreted (according to Swiss-Prot)
Database entries
- Structure:
- UniProt: P54482
- KEGG entry: [3]
- E.C. number:
Additional information
Expression and regulation
- Additional information:
Biological materials
- Mutant:
- Expression vector:
- lacZ fusion:
- GFP fusion:
- two-hybrid system:
- Antibody:
Labs working on this gene/protein
Your additional remarks
References
Kazuya Nakagawa, Kentaro Takada, Nobutaka Imamura
Probable novel MEP pathway inhibitor and its binding protein, IspG.
Biosci Biotechnol Biochem: 2013, 77(7);1449-54
[PubMed:23832336]
[WorldCat.org]
[DOI]
(I p)
Mattijs K Julsing, Michael Rijpkema, Herman J Woerdenbag, Wim J Quax, Oliver Kayser
Functional analysis of genes involved in the biosynthesis of isoprene in Bacillus subtilis.
Appl Microbiol Biotechnol: 2007, 75(6);1377-84
[PubMed:17458547]
[WorldCat.org]
[DOI]
(P p)
Patrick Eichenberger, Masaya Fujita, Shane T Jensen, Erin M Conlon, David Z Rudner, Stephanie T Wang, Caitlin Ferguson, Koki Haga, Tsutomu Sato, Jun S Liu, Richard Losick
The program of gene transcription for a single differentiating cell type during sporulation in Bacillus subtilis.
PLoS Biol: 2004, 2(10);e328
[PubMed:15383836]
[WorldCat.org]
[DOI]
(I p)
Patrick Eichenberger, Shane T Jensen, Erin M Conlon, Christiaan van Ooij, Jessica Silvaggi, José Eduardo González-Pastor, Masaya Fujita, Sigal Ben-Yehuda, Patrick Stragier, Jun S Liu, Richard Losick
The sigmaE regulon and the identification of additional sporulation genes in Bacillus subtilis.
J Mol Biol: 2003, 327(5);945-72
[PubMed:12662922]
[WorldCat.org]
[DOI]
(P p)
