Difference between revisions of "IspG"

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=Expression and regulation=
 
=Expression and regulation=
 +
* '''Operon:''' ''[[yqfZ]]-[[yqfY]]'' {{PubMed|12662922}}
  
* '''Operon:'''
+
* '''[[Sigma factor]]:''' [[SigE]] {{PubMed|12662922}}
 
 
* '''Sigma factor:'''  
 
  
 
* '''Regulation:'''  
 
* '''Regulation:'''  
 +
** expressed early during sporulation in the mother cell ([[SigE]], [[SpoIIID]]) {{PubMed|12662922,15383836}}
  
 
* '''Regulatory mechanism:'''  
 
* '''Regulatory mechanism:'''  
 
+
** [[SpoIIID]]: transcription repression {{PubMed|15383836}}
 +
 
* '''Additional information:'''  
 
* '''Additional information:'''  
  
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=References=
 
=References=
<pubmed> 17458547 </pubmed>
+
<pubmed>12662922 15383836 17458547 </pubmed>
  
 
[[Category:Protein-coding genes]]
 
[[Category:Protein-coding genes]]

Revision as of 17:38, 22 November 2009

  • Description: similar to peptidoglycan acetylation, 1-hydroxy-2-methyl-2-(E)-butenyl-4-diphosphate synthase

Gene name ispG
Synonyms yqfY
Essential yes PubMed
Product unknown
Function unknown
MW, pI 40 kDa, 6.802
Gene length, protein length 1131 bp, 377 aa
Immediate neighbours yqfZ, yqfX
Get the DNA and protein sequences
(Barbe et al., 2009)
Genetic context
YqfY context.gif
This image was kindly provided by SubtiList



The gene

Basic information

  • Locus tag: BSU25070

Phenotypes of a mutant

essential PubMed

Database entries

  • DBTBS entry: [1]
  • SubtiList entry: [2]

Additional information

The protein

Basic information/ Evolution

  • Catalyzed reaction/ biological activity: (E)-4-hydroxy-3-methylbut-2-en-1-yl diphosphate + H2O + protein-disulfide = 2-C-methyl-D-erythritol 2,4-cyclodiphosphate + protein-dithiol (according to Swiss-Prot)
  • Protein family: ispG family (according to Swiss-Prot)
  • Paralogous protein(s):

Extended information on the protein

  • Kinetic information:
  • Domains:
  • Modification:
  • Cofactor(s):
  • Effectors of protein activity:
  • Interactions:
  • Localization: secreted (according to Swiss-Prot)

Database entries

  • Structure:
  • KEGG entry: [3]
  • E.C. number:

Additional information

Expression and regulation

  • Regulation:
  • Additional information:

Biological materials

  • Mutant:
  • Expression vector:
  • lacZ fusion:
  • GFP fusion:
  • two-hybrid system:
  • Antibody:

Labs working on this gene/protein

Your additional remarks

References

Mattijs K Julsing, Michael Rijpkema, Herman J Woerdenbag, Wim J Quax, Oliver Kayser
Functional analysis of genes involved in the biosynthesis of isoprene in Bacillus subtilis.
Appl Microbiol Biotechnol: 2007, 75(6);1377-84
[PubMed:17458547] [WorldCat.org] [DOI] (P p)

Patrick Eichenberger, Masaya Fujita, Shane T Jensen, Erin M Conlon, David Z Rudner, Stephanie T Wang, Caitlin Ferguson, Koki Haga, Tsutomu Sato, Jun S Liu, Richard Losick
The program of gene transcription for a single differentiating cell type during sporulation in Bacillus subtilis.
PLoS Biol: 2004, 2(10);e328
[PubMed:15383836] [WorldCat.org] [DOI] (I p)

Patrick Eichenberger, Shane T Jensen, Erin M Conlon, Christiaan van Ooij, Jessica Silvaggi, José Eduardo González-Pastor, Masaya Fujita, Sigal Ben-Yehuda, Patrick Stragier, Jun S Liu, Richard Losick
The sigmaE regulon and the identification of additional sporulation genes in Bacillus subtilis.
J Mol Biol: 2003, 327(5);945-72
[PubMed:12662922] [WorldCat.org] [DOI] (P p)