Difference between revisions of "IspF"

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* '''Description:''' 2-C-methyl-D-erythrol-2,4-cyclodiphosphate synthase <br/><br/>
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* '''Description:''' 2-C-methyl-D-erythrol-2,4-cyclodiphosphate synthase, 5th step in the MEP pathway of isoprenoid biosynthesis <br/><br/>
  
 
{| align="right" border="1" cellpadding="2"  
 
{| align="right" border="1" cellpadding="2"  
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|style="background:#ABCDEF;" align="center"| '''Product''' || 2-C-methyl-D-erythrol-2,4-cyclodiphosphate synthase  
 
|style="background:#ABCDEF;" align="center"| '''Product''' || 2-C-methyl-D-erythrol-2,4-cyclodiphosphate synthase  
 
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|style="background:#ABCDEF;" align="center"|'''Function''' || isoprenoid biosynthesis
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|style="background:#ABCDEF;" align="center"|'''Function''' || MEP pathway of isoprenoid biosynthesis
 
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|colspan="2" style="background:#FAF8CC;" align="center"| '''Gene expression levels in [http://subtiwiki.uni-goettingen.de/apps/expression/ ''Subti''Express]''': [http://subtiwiki.uni-goettingen.de/apps/expression/expression.php?search=BSU00910 ispF]
 
|colspan="2" style="background:#FAF8CC;" align="center"| '''Gene expression levels in [http://subtiwiki.uni-goettingen.de/apps/expression/ ''Subti''Express]''': [http://subtiwiki.uni-goettingen.de/apps/expression/expression.php?search=BSU00910 ispF]
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* '''Expression browser:''' [http://genome.jouy.inra.fr/cgi-bin/seb/viewdetail.py?id=ispF_110480_110956_1 ispF] {{PubMed|22383849}}
 
* '''Expression browser:''' [http://genome.jouy.inra.fr/cgi-bin/seb/viewdetail.py?id=ispF_110480_110956_1 ispF] {{PubMed|22383849}}
  
* '''Sigma factor:''' [[SigB]] [http://www.ncbi.nlm.nih.gov/pubmed/15805528 PubMed]  
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* '''[[Sigma factor]]:''' [[SigB]] [http://www.ncbi.nlm.nih.gov/pubmed/15805528 PubMed]  
  
 
* '''Regulation:'''  induced by stress ([[SigB]]) [http://www.ncbi.nlm.nih.gov/pubmed/15805528 PubMed]
 
* '''Regulation:'''  induced by stress ([[SigB]]) [http://www.ncbi.nlm.nih.gov/pubmed/15805528 PubMed]

Revision as of 08:09, 9 July 2013

  • Description: 2-C-methyl-D-erythrol-2,4-cyclodiphosphate synthase, 5th step in the MEP pathway of isoprenoid biosynthesis

Gene name ispF
Synonyms yacN
Essential yes PubMed
Product 2-C-methyl-D-erythrol-2,4-cyclodiphosphate synthase
Function MEP pathway of isoprenoid biosynthesis
Gene expression levels in SubtiExpress: ispF
MW, pI 16 kDa, 5.398
Gene length, protein length 474 bp, 158 aa
Immediate neighbours ispD, gltX
Sequences Protein DNA DNA_with_flanks
Genetic context
YacN context.gif
This image was kindly provided by SubtiList
Expression at a glance   PubMed
IspF expression.png















Categories containing this gene/protein

biosynthesis of lipids, general stress proteins (controlled by SigB), essential genes

This gene is a member of the following regulons

SigB regulon

The gene

Basic information

  • Locus tag: BSU00910

Phenotypes of a mutant

essential PubMed

Database entries

  • DBTBS entry: [1]
  • SubtiList entry: [2]

Additional information

The protein

Basic information/ Evolution

  • Catalyzed reaction/ biological activity: 2-phospho-4-(cytidine 5'-diphospho)-2-C-methyl-D-erythritol = 2-C-methyl-D-erythritol 2,4-cyclodiphosphate + CMP (according to Swiss-Prot)
  • Protein family: ispF family (according to Swiss-Prot)
  • Paralogous protein(s):

Extended information on the protein

  • Kinetic information:
  • Domains:
  • Modification:
  • Cofactor(s):
  • Effectors of protein activity:

Database entries

  • Structure:
  • KEGG entry: [3]

Additional information

Expression and regulation

  • Regulatory mechanism:
  • Additional information:

Biological materials

  • Mutant:
  • Expression vector:
  • lacZ fusion:
  • GFP fusion:
  • two-hybrid system:
  • Antibody:

Labs working on this gene/protein

Your additional remarks

References