Difference between revisions of "IspF"

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|style="background:#ABCDEF;" align="center"|'''Function''' || isoprenoid biosynthesis
 
|style="background:#ABCDEF;" align="center"|'''Function''' || isoprenoid biosynthesis
 
|-
 
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|colspan="2" style="background:#FAF8CC;" align="center"| '''Gene expression levels in [http://cellpublisher.gobics.de/subtiexpress/ ''Subti''Express]''': [http://cellpublisher.gobics.de/subtiexpress/bsu/BSU00910 ispF]
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|colspan="2" style="background:#FAF8CC;" align="center"| '''Gene expression levels in [http://subtiwiki.uni-goettingen.de/apps/expression/ ''Subti''Express]''': [http://subtiwiki.uni-goettingen.de/apps/expression/expression.php?search=BSU00910 ispF]
 
|-
 
|-
 
|style="background:#ABCDEF;" align="center"| '''MW, pI''' || 16 kDa, 5.398   
 
|style="background:#ABCDEF;" align="center"| '''MW, pI''' || 16 kDa, 5.398   
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|style="background:#ABCDEF;" align="center"|'''Immediate neighbours''' || ''[[ispD]]'', ''[[gltX]]''
 
|style="background:#ABCDEF;" align="center"|'''Immediate neighbours''' || ''[[ispD]]'', ''[[gltX]]''
 
|-
 
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|colspan="2" style="background:#FAF8CC;" align="center"|'''Get the DNA and protein [http://srs.ebi.ac.uk/srsbin/cgi-bin/wgetz?-e+&#91;EMBLCDS:CAB11867&#93;+-newId sequences] <br/> (Barbe ''et al.'', 2009)'''
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|style="background:#FAF8CC;" align="center"|'''Sequences'''||[http://bsubcyc.org/BSUB/sequence-aa?type=GENE&object=BSU00910 Protein] [http://bsubcyc.org/BSUB/sequence?type=GENE&object=BSU00910 DNA] [http://bsubcyc.org/BSUB/seq-selector?chromosome=CHROM-1&object=BSU00910 Advanced_DNA]
 
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|-
 
|colspan="2" | '''Genetic context''' <br/> [[Image:yacN_context.gif]]
 
|colspan="2" | '''Genetic context''' <br/> [[Image:yacN_context.gif]]

Revision as of 11:57, 13 May 2013

  • Description: 2-C-methyl-D-erythrol-2,4-cyclodiphosphate synthase

Gene name ispF
Synonyms yacN
Essential yes PubMed
Product 2-C-methyl-D-erythrol-2,4-cyclodiphosphate synthase
Function isoprenoid biosynthesis
Gene expression levels in SubtiExpress: ispF
MW, pI 16 kDa, 5.398
Gene length, protein length 474 bp, 158 aa
Immediate neighbours ispD, gltX
Sequences Protein DNA Advanced_DNA
Genetic context
YacN context.gif
This image was kindly provided by SubtiList
Expression at a glance   PubMed
IspF expression.png
























Categories containing this gene/protein

biosynthesis of lipids, general stress proteins (controlled by SigB), essential genes

This gene is a member of the following regulons

SigB regulon

The gene

Basic information

  • Locus tag: BSU00910

Phenotypes of a mutant

essential PubMed

Database entries

  • DBTBS entry: [1]
  • SubtiList entry: [2]

Additional information

The protein

Basic information/ Evolution

  • Catalyzed reaction/ biological activity: 2-phospho-4-(cytidine 5'-diphospho)-2-C-methyl-D-erythritol = 2-C-methyl-D-erythritol 2,4-cyclodiphosphate + CMP (according to Swiss-Prot)
  • Protein family: ispF family (according to Swiss-Prot)
  • Paralogous protein(s):

Extended information on the protein

  • Kinetic information:
  • Domains:
  • Modification:
  • Cofactor(s):
  • Effectors of protein activity:

Database entries

  • Structure:
  • KEGG entry: [3]

Additional information

Expression and regulation

  • Regulatory mechanism:
  • Additional information:

Biological materials

  • Mutant:
  • Expression vector:
  • lacZ fusion:
  • GFP fusion:
  • two-hybrid system:
  • Antibody:

Labs working on this gene/protein

Your additional remarks

References

Mattijs K Julsing, Michael Rijpkema, Herman J Woerdenbag, Wim J Quax, Oliver Kayser
Functional analysis of genes involved in the biosynthesis of isoprene in Bacillus subtilis.
Appl Microbiol Biotechnol: 2007, 75(6);1377-84
[PubMed:17458547] [WorldCat.org] [DOI] (P p)

Dirk Höper, Uwe Völker, Michael Hecker
Comprehensive characterization of the contribution of individual SigB-dependent general stress genes to stress resistance of Bacillus subtilis.
J Bacteriol: 2005, 187(8);2810-26
[PubMed:15805528] [WorldCat.org] [DOI] (P p)

Tracey L Campbell, Eric D Brown
Characterization of the depletion of 2-C-methyl-D-erythritol-2,4-cyclodiphosphate synthase in Escherichia coli and Bacillus subtilis.
J Bacteriol: 2002, 184(20);5609-18
[PubMed:12270818] [WorldCat.org] [DOI] (P p)

A Petersohn, J Bernhardt, U Gerth, D Höper, T Koburger, U Völker, M Hecker
Identification of sigma(B)-dependent genes in Bacillus subtilis using a promoter consensus-directed search and oligonucleotide hybridization.
J Bacteriol: 1999, 181(18);5718-24
[PubMed:10482513] [WorldCat.org] [DOI] (P p)