IspA

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  • Description: intracellular serine protease

Gene name ispA
Synonyms
Essential no
Product intracellular serine protease
Function protein degradation
Gene expression levels in SubtiExpress: ispA
MW, pI 33 kDa, 4.522
Gene length, protein length 957 bp, 319 aa
Immediate neighbours metE, rsbRB
Sequences Protein DNA DNA_with_flanks
Genetic context
IspA context.gif
This image was kindly provided by SubtiList
Expression at a glance   PubMed
IspA expression.png















Categories containing this gene/protein

utilization of nitrogen sources other than amino acids, proteolysis

This gene is a member of the following regulons

CodY regulon

The gene

Basic information

  • Locus tag: BSU13190

Phenotypes of a mutant

Database entries

  • DBTBS entry: [1]
  • SubtiList entry: [2]

Additional information

The protein

Basic information/ Evolution

  • Catalyzed reaction/ biological activity:
  • Protein family: peptidase S8 family (according to Swiss-Prot)
  • Paralogous protein(s):

Extended information on the protein

  • Kinetic information:
  • Domains:
  • Modification:
  • Cofactor(s):
  • Effectors of protein activity:

Database entries

  • Structure:
  • KEGG entry: [3]
  • E.C. number:

Additional information

Expression and regulation

  • Operon: ispA (according to DBTBS)
  • Regulation:
    • repressed during growth in the presence of branched chain amino acids (CodY) PubMed
  • Regulatory mechanism:
  • Additional information:

Biological materials

  • Mutant:
  • Expression vector:
  • lacZ fusion:
  • GFP fusion:
  • two-hybrid system:
  • Antibody:

Labs working on this gene/protein

Your additional remarks

References

Boris R Belitsky, Abraham L Sonenshein
Genome-wide identification of Bacillus subtilis CodY-binding sites at single-nucleotide resolution.
Proc Natl Acad Sci U S A: 2013, 110(17);7026-31
[PubMed:23569278] [WorldCat.org] [DOI] (I p)

Y Koide, A Nakamura, T Uozumi, T Beppu
Cloning and sequencing of the major intracellular serine protease gene of Bacillus subtilis.
J Bacteriol: 1986, 167(1);110-6
[PubMed:3087947] [WorldCat.org] [DOI] (P p)