rodA

168

SEDS family peptidoglycan glycosyltransferase, part of the [wiki|elongasome] for lateral [wiki|cell wall synthesis] and control of cell diameter

locus: BSU_38120

Molecular weight: 43.12 kDa

pI: 8.76

Protein length: 393 aa

Gene length: 1182 bp

function: control of cell shape and elongation

product: SEDS family peptidoglycan glycosyltransferase

essential: yes

synonyms: rodA, ywcF, ipa-42d

List of homologs in different organisms, belongs to COG0772 (Galperin et al., 2021)

Gene

Coordinates: 3,912,332 → 3,913,513

Phenotypes of a mutant

• essential [Pubmed|12682299]
• CRISPRi knockdown mutant has a terminal phenotype of severe lysis [Pubmed|27238023]

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The protein

Catalyzed reaction/ biological activity

• principal peptidoglycan glycosyltransferase of the [wiki|elongasome] [Pubmed|27525505]
• [GlcNAc-(1→4)-Mur2Ac(oyl-L-Ala-γ-D-Glu-L-Lys-D-Ala-D-Ala)]_(n)-diphospho-di-trans,octa-cis-undecaprenol + β-D-GlcNAc-(1→4)-Mur2Ac(oyl-L-Ala-γ-D-Glu-L-Lys-D-Ala-D-Ala)-diphospho-di-trans,octa-cis-undecaprenol --> [GlcNAc-(1→4)-Mur2Ac(oyl-L-Ala-γ-D-Glu-L-Lys-D-Ala-D-Ala)]_(n+1)-diphospho-di-trans-octa-cis-undecaprenol + di-trans,octa-cis-undecaprenyl diphosphate + H⁺ (according to UniProt)

Protein family

• [wiki|SEDS proteins] (shape, elongation, division, and sporulation) [Pubmed|27525505]
• SEDS family (with [protein|8AB7D225DBEE6BD695A4A8A2384D2C029B571C57|ftsW] and [protein|5DE602D97D903D9AE38ED05D5A5F1B3A2DC1D58E|spoVE], according to UniProt)

Structure

• [PDB|6BAR] (from Thermus thermophilus, 26% identity) [pubmed|29590088]
• [AF|P39604]

Paralogous protein(s)

• [protein|5DE602D97D903D9AE38ED05D5A5F1B3A2DC1D58E|spoVE], [protein|8AB7D225DBEE6BD695A4A8A2384D2C029B571C57|ftsW]

[wiki|Localization]

• integral membrane protein [Pubmed|21636744]
• forms circumferentially moving patches [Pubmed|21636744]
• localization depends on [protein|A4C8719E06F774A6EB4D79757CC79CF89E453A54|mreB] [pubmed|26679002]

Expression and Regulation

Operons

• genes: [gene|B405B3C21B464F904BBB2AFD5DA21ADE45B4DD96|rodA]

  description: [Pubmed|9353933]

  sigma factors

  • [protein|081DF3EE9FA56209D648C7677188C61CE3AA8E41|sigM]: sigma factor, [Pubmed|18179421], in [regulon|protein:081DF3EE9FA56209D648C7677188C61CE3AA8E41|sigM regulon]

Biological materials

Mutant

• xylose-inducible CRISPRi knockdown mutant BEC38120 is available from the [http://subtiwiki.uni-goettingen.de/wiki/index.php/Daniel_R._Zeigler BGSC]

labs

• [wiki|Charles Moran], Emory University, NC, USA [http://www.microbiology.emory.edu/moran_c.html homepage]
• [wiki|David Rudner], Harvard Medical School, MA, USA [http://rudnerlab.med.harvard.edu/ homepage]

References

Reviews

• 26679002,28975672,33709487

Original Publications

• 27525505,27238023,9622350,21636744,21636745,17981970,28085152,29590088,31086310,32152588,32973704,37333659