Difference between revisions of "IolB"

From SubtiWiki
Jump to: navigation, search
Line 32: Line 32:
  
 
<br/><br/><br/><br/><br/><br/>
 
<br/><br/><br/><br/><br/><br/>
 +
 +
= [[Categories]] containing this gene/protein =
 +
{{SubtiWiki category|[[utilization of specific carbon sources]]}}
 +
 +
= This gene is a member of the following [[regulons]] =
 +
{{SubtiWiki regulon|[[CcpA regulon]]}},
 +
{{SubtiWiki regulon|[[IolR regulon]]}}
  
 
=The gene=
 
=The gene=
Line 51: Line 58:
  
  
= Categories containing this gene/protein =
+
 
{{SubtiWiki category|[[utilization of specific carbon sources]]}}
 
 
=The protein=
 
=The protein=
  

Revision as of 01:05, 9 December 2010

  • Description: formation of 2-deoxy-5-keto-gluconic acid (4th reaction)

Gene name iolB
Synonyms yxdB
Essential no
Product formation of 2-deoxy-5-keto-gluconic acid (4th reaction)
Function myo-inositol catabolism
Metabolic function and regulation of this protein in SubtiPathways:
Sugar catabolism
MW, pI 30 kDa, 4.77
Gene length, protein length 813 bp, 271 aa
Immediate neighbours iolC, iolA
Get the DNA and protein sequences
(Barbe et al., 2009)
Genetic context
IolB context.gif
This image was kindly provided by SubtiList







Categories containing this gene/protein

utilization of specific carbon sources

This gene is a member of the following regulons

CcpA regulon, IolR regulon

The gene

Basic information

  • Locus tag: BSU39750

Phenotypes of a mutant

Database entries

  • DBTBS entry: [1]
  • SubtiList entry: [2]

Additional information

The protein

Basic information/ Evolution

  • Catalyzed reaction/ biological activity: 5-deoxy-glucuronic acid = 5-dehydro-2-deoxy-D-gluconic acid (according to Swiss-Prot)
  • Protein family: isomerase iolB family (according to Swiss-Prot)
  • Paralogous protein(s):

Extended information on the protein

  • Kinetic information:
  • Domains:
  • Modification:
  • Cofactor(s):
  • Effectors of protein activity:
  • Interactions:
  • Localization:

Database entries

  • Structure: 2QJV (from Salmonella typhimurium, 49% identity, 65% similarity)
  • KEGG entry: [3]
  • E.C. number:

Additional information

Expression and regulation

  • Additional information:

Biological materials

  • Mutant:
  • Expression vector:
  • lacZ fusion:
  • GFP fusion:
  • two-hybrid system:
  • Antibody:

Labs working on this gene/protein

Your additional remarks

References

Ken-ichi Yoshida, Masanori Yamaguchi, Tetsuro Morinaga, Masaki Kinehara, Maya Ikeuchi, Hitoshi Ashida, Yasutaro Fujita
myo-Inositol catabolism in Bacillus subtilis.
J Biol Chem: 2008, 283(16);10415-24
[PubMed:18310071] [WorldCat.org] [DOI] (P p)

Y Miwa, Y Fujita
Involvement of two distinct catabolite-responsive elements in catabolite repression of the Bacillus subtilis myo-inositol (iol) operon.
J Bacteriol: 2001, 183(20);5877-84
[PubMed:11566986] [WorldCat.org] [DOI] (P p)

K I Yoshida, T Shibayama, D Aoyama, Y Fujita
Interaction of a repressor and its binding sites for regulation of the Bacillus subtilis iol divergon.
J Mol Biol: 1999, 285(3);917-29
[PubMed:9887260] [WorldCat.org] [DOI] (P p)

K I Yoshida, D Aoyama, I Ishio, T Shibayama, Y Fujita
Organization and transcription of the myo-inositol operon, iol, of Bacillus subtilis.
J Bacteriol: 1997, 179(14);4591-8
[PubMed:9226270] [WorldCat.org] [DOI] (P p)