InfB

From SubtiWiki
Revision as of 12:52, 13 May 2013 by 134.76.70.252 (talk)
Jump to: navigation, search
  • Description: translation initiation factor IF-2

Gene name infB
Synonyms
Essential yes PubMed
Product initiation factor IF-2
Function translation
Gene expression levels in SubtiExpress: infB
MW, pI 78 kDa, 5.191
Gene length, protein length 2148 bp, 716 aa
Immediate neighbours ylxQ, ylxP
Sequences Protein DNA Advanced_DNA
Genetic context
InfB context.gif
This image was kindly provided by SubtiList
Expression at a glance   PubMed
InfB expression.png
























Categories containing this gene/protein

translation, essential genes

This gene is a member of the following regulons

stringent response

The gene

Basic information

  • Locus tag: BSU16630

Phenotypes of a mutant

essential PubMed

Database entries

  • DBTBS entry: [1]
  • SubtiList entry: [2]

Additional information

The protein

Basic information/ Evolution

  • Catalyzed reaction/ biological activity: Initiation of translation
  • Protein family: IF-2 family (according to Swiss-Prot)
  • Paralogous protein(s):

Extended information on the protein

  • Kinetic information:
  • Domains: Nucleotide-binding domain that competitively binds GTP or ppGpp PubMed
  • Modification:
  • Cofactor(s):
  • Effectors of protein activity: GTP and ppGpp PubMed

Database entries

  • Structure: 1D1N (fMet-tRNA-fMet binding domain, Geobacillus stearothermophilus), 1Z9B (C1-subdomain, Geobacillus stearothermophilus)
  • KEGG entry: [3]
  • E.C. number:

Additional information

Expression and regulation

  • Regulation:
    • RelA dependent downregulation (Class I) during stringent response PubMed
    • Interaction with ppGpp interferes with IF2-dependent initiation complex formation, severely inhibits initiation dipeptide formation, and blocks the initiation step of translation.PubMed
  • Regulatory mechanism:
  • Additional information: Putative cellular metabolic sensor and regulator that oscillates between an active GTP-bound form under conditions allowing active protein syntheses and an inactive ppGpp-bound form when depleting of nutrients PubMed

Biological materials

  • Mutant:
  • Expression vector:
  • lacZ fusion:
  • GFP fusion:
  • two-hybrid system:
  • Antibody:

Labs working on this gene/protein

Your additional remarks

References