infB

168

translation initiation factor IF-2, ribosome-dependent GTPase interacting with 30 ribosome subunits, ensures correct binding of initiator tRNAs

locus: BSU_16630

Molecular weight: 78.35 kDa

pI: 5.19

Protein length: 716 aa

Gene length: 2151 bp

function: translation

product: initiation factor IF-2

essential: yes

synonyms: infB

List of homologs in different organisms, belongs to COG0532 (Galperin et al., 2021)

Gene

Coordinates: 1,734,009  1,736,159

Phenotypes of a mutant

• essential [Pubmed|12682299]

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The protein

Catalyzed reaction/ biological activity

• ribosome-dependent GTPase interacting with 30 ribosome subunits, ensures correct binding of initiator tRNAs [pubmed|15755955,26259514]
• Putative cellular metabolic sensor and regulator that oscillates between an active GTP-bound form under conditions allowing active protein syntheses and an inactive ppGpp-bound form when depleting of nutrients [PubMed|16968770]

Protein family

• [wiki|TRAFAC class translation factor GTPase superfamily] (according to UniProt)
• [wiki|Classic translation factor GTPase family] (according to UniProt)

[wiki|Domains]

• Nucleotide-binding domain that competitively binds GTP or ppGpp [Pubmed|16968770]
• tr-type G domain (aa 217-386) (according to UniProt)

Structure

• [PDB|1D1N] (fMet-tRNA-fMet binding domain, Geobacillus stearothermophilus),  [PDB|1Z9B] (C1-subdomain, Geobacillus stearothermophilus)
• [AF|P17889]

Effectors of protein activity

• GTP and ppGpp [Pubmed|16968770]

[wiki|Localization]

• cytoplasm (according to UniProt)

References

Reviews

• 15755955,26259514

Original Publications

• 36815589,11948165,8491709,16968770,32576694