Difference between revisions of "IlvH"

From SubtiWiki
Jump to: navigation, search
m (Reverted edits by 134.76.70.252 (talk) to last revision by Jstuelk)
Line 47: Line 47:
  
 
* '''Locus tag:''' BSU28300
 
* '''Locus tag:''' BSU28300
 
[http://genome.jouy.inra.fr/cgi-bin/seb/viewdetail.py?id=ilvH_2894733_2895251_-1 Expression]
 
  
 
===Phenotypes of a mutant ===
 
===Phenotypes of a mutant ===

Revision as of 11:31, 26 January 2012

  • Description: acetolactate synthase (large subunit)

Gene name ilvH
Synonyms ilvN
Essential no
Product acetolactate synthase (large subunit)
Function biosynthesis of branched-chain amino acids
Metabolic function and regulation of this protein in SubtiPathways:
Ile, Leu, Val, Coenzyme A
MW, pI 19 kDa, 10.295
Gene length, protein length 522 bp, 174 aa
Immediate neighbours ilvC, ilvB
Get the DNA and protein sequences
(Barbe et al., 2009)
Genetic context
IlvH context.gif
This image was kindly provided by SubtiList







Categories containing this gene/protein

biosynthesis/ acquisition of amino acids

This gene is a member of the following regulons

CcpA regulon, CodY regulon, T-box, TnrA regulon

The gene

Basic information

  • Locus tag: BSU28300

Phenotypes of a mutant

Database entries

  • DBTBS entry: [1]
  • SubtiList entry: [2]

Additional information

The protein

Basic information/ Evolution

  • Catalyzed reaction/ biological activity: 2 pyruvate = 2-acetolactate + CO2 (according to Swiss-Prot)
  • Protein family: acetolactate synthase small subunit family (according to Swiss-Prot)
  • Paralogous protein(s):

Extended information on the protein

  • Kinetic information:
  • Domains:
  • Modification:
  • Cofactor(s):
  • Effectors of protein activity:

Database entries

  • Structure: 2F1F (regulatory subunit from Escherichia coli, 37% identity, 62% similarity) PubMed
  • KEGG entry: [3]

Additional information

Expression and regulation

  • Regulation: for a complete overview on the regulation of the ilv operon, see Brinsmade et al.
    • repressed by casamino acids PubMed
    • expression is stimulated in the presence of glucose PubMed
    • repressed in the absence of good nitrogen sources (glutamine or ammonium) (TnrA) PubMed
    • repressed during growth in the presence of branched chain amino acids (CodY) PubMed
  • Regulatory mechanism:
    • glucose regulation: CcpA PubMed
    • repression by BCAA: tRNA-controlled RNA switch (T-box) that mediates termination/antitermination
    • TnrA: transcription repression PubMed
    • CodY: transcription repression PubMed
  • Additional information:

Biological materials

  • Mutant:
  • Expression vector:
  • lacZ fusion:
  • GFP fusion:
  • two-hybrid system:
  • Antibody:

Labs working on this gene/protein

Your additional remarks

References