HutH

• Description: histidase
  
  

Gene name	hutH
Synonyms
Essential	no
Product	histidase
Function	histidine utilization
Metabolic function and regulation of this protein in SubtiPathways: His
MW, pI	55 kDa, 5.221
Gene length, protein length	1524 bp, 508 aa
Immediate neighbours	hutP, hutU
Get the DNA and protein sequences (Barbe et al., 2009)
Genetic context This image was kindly provided by SubtiList

The gene

Basic information

• Locus tag: BSU39350

Database entries

• DBTBS entry: [1]

• SubtiList entry: [2]

Categories containing this gene/protein

utilization of amino acids

The protein

Basic information/ Evolution

• Catalyzed reaction/ biological activity: L-histidine = urocanate + NH₃ (according to Swiss-Prot)

• Protein family: PAL/histidase family (according to Swiss-Prot)

• Paralogous protein(s):

Extended information on the protein

• Kinetic information:

• Domains:

• Modification:

• Cofactor(s):

• Effectors of protein activity:

• Interactions:

• Localization: cytoplasm (according to Swiss-Prot)

Database entries

• Structure: 1B8F (from Pseudomonas putida, 43% identity, 62% similarity) PubMed

• UniProt: P10944

• KEGG entry: [3]

• E.C. number: 4.3.1.3

Expression and regulation

• Operon: hutP-hutH-hutU-hutI-hutG-hutM PubMed

• Sigma factor: SigA PubMed

• Regulation:
  • repressed by glucose (CcpA) PubMed
  • induced by histidine (HutP) PubMed
  • repressed during growth in the presence of branched chain amino acids (CodY) PubMed

• Regulatory mechanism:
  • CcpA: transcription repression PubMed
  • CodY: transcription repression PubMed
  • HutP: transcriptional antitermination at a protein-dependent RNA switch located between hutP and hutH PubMed

• Additional information:

Biological materials

• Mutant: GP1114 (spc), available in Stülke lab

• Expression vector:

• lacZ fusion:

• GFP fusion:

• two-hybrid system:

• Antibody: