Difference between revisions of "HutH"

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= [[Categories]] containing this gene/protein =
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{{SubtiWiki category|[[utilization of amino acids]]}}
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= This gene is a member of the following [[regulons]] =
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{{SubtiWiki regulon|[[AbrB regulon]]}},
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{{SubtiWiki regulon|[[CcpA regulon]]}},
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{{SubtiWiki regulon|[[CodY regulon]]}},
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{{SubtiWiki regulon|[[HutP regulon]]}}
  
 
=The gene=
 
=The gene=
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= Categories containing this gene/protein =
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{{SubtiWiki category|[[utilization of amino acids]]}}
 
 
=The protein=
 
=The protein=
  

Revision as of 00:57, 9 December 2010

  • Description: histidase

Gene name hutH
Synonyms
Essential no
Product histidase
Function histidine utilization
Metabolic function and regulation of this protein in SubtiPathways:
His
MW, pI 55 kDa, 5.221
Gene length, protein length 1524 bp, 508 aa
Immediate neighbours hutP, hutU
Get the DNA and protein sequences
(Barbe et al., 2009)
Genetic context
HutH context.gif
This image was kindly provided by SubtiList







Categories containing this gene/protein

utilization of amino acids

This gene is a member of the following regulons

AbrB regulon, CcpA regulon, CodY regulon, HutP regulon

The gene

Basic information

  • Locus tag: BSU39350

Phenotypes of a mutant

Database entries

  • DBTBS entry: [1]
  • SubtiList entry: [2]

Additional information

The protein

Basic information/ Evolution

  • Catalyzed reaction/ biological activity: L-histidine = urocanate + NH3 (according to Swiss-Prot)
  • Protein family: PAL/histidase family (according to Swiss-Prot)
  • Paralogous protein(s):

Extended information on the protein

  • Kinetic information:
  • Domains:
  • Modification:
  • Cofactor(s):
  • Effectors of protein activity:
  • Interactions:
  • Localization: cytoplasm (according to Swiss-Prot)

Database entries

  • Structure: 1B8F (from Pseudomonas putida, 43% identity, 62% similarity) PubMed
  • KEGG entry: [3]

Additional information

Expression and regulation

  • Regulation:
    • repressed by glucose (CcpA) PubMed
    • induced by histidine (HutP) PubMed
    • repressed during growth in the presence of branched chain amino acids (CodY) PubMed
  • Additional information:

Biological materials

  • Mutant: GP1114 (spc), available in Stülke lab
  • Expression vector:
  • lacZ fusion:
  • GFP fusion:
  • two-hybrid system:
  • Antibody:

Labs working on this gene/protein

Your additional remarks

References