Difference between revisions of "HtpX"

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|style="background:#ABCDEF;" align="center"|'''Function''' || quality control of membrane proteins
 
|style="background:#ABCDEF;" align="center"|'''Function''' || quality control of membrane proteins
 
|-
 
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|colspan="2" style="background:#FAF8CC;" align="center"| '''Gene expression levels in [http://cellpublisher.gobics.de/subtiexpress/ ''Subti''Express]''': [http://cellpublisher.gobics.de/subtiexpress/bsu/BSU13490 htpX]
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|colspan="2" style="background:#FAF8CC;" align="center"| '''Gene expression levels in [http://subtiwiki.uni-goettingen.de/apps/expression/ ''Subti''Express]''': [http://subtiwiki.uni-goettingen.de/apps/expression/expression.php?search=BSU13490 htpX]
 
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|style="background:#ABCDEF;" align="center"| '''MW, pI''' || 32 kDa, 10.157   
 
|style="background:#ABCDEF;" align="center"| '''MW, pI''' || 32 kDa, 10.157   
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|style="background:#ABCDEF;" align="center"|'''Immediate neighbours''' || ''[[ykrK]]'', ''[[ktrD]]''
 
|style="background:#ABCDEF;" align="center"|'''Immediate neighbours''' || ''[[ykrK]]'', ''[[ktrD]]''
 
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|colspan="2" style="background:#FAF8CC;" align="center"|'''Get the DNA and protein [http://srs.ebi.ac.uk/srsbin/cgi-bin/wgetz?-e+&#91;EMBLCDS:CAB13222&#93;+-newId sequences] <br/> (Barbe ''et al.'', 2009)'''
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|style="background:#FAF8CC;" align="center"|'''Sequences'''||[http://bsubcyc.org/BSUB/sequence-aa?type=GENE&object=BSU13490 Protein] [http://bsubcyc.org/BSUB/sequence?type=GENE&object=BSU13490 DNA] [http://bsubcyc.org/BSUB/seq-selector?chromosome=CHROM-1&object=BSU13490 Advanced_DNA]
 
|-
 
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|colspan="2" | '''Genetic context''' <br/> [[Image:ykrL_context.gif]]
 
|colspan="2" | '''Genetic context''' <br/> [[Image:ykrL_context.gif]]

Revision as of 12:39, 13 May 2013

  • Description: stress-responsive membrane protease

Gene name htpX
Synonyms ykrL
Essential no
Product membrane protease
Function quality control of membrane proteins
Gene expression levels in SubtiExpress: htpX
MW, pI 32 kDa, 10.157
Gene length, protein length 894 bp, 298 aa
Immediate neighbours ykrK, ktrD
Sequences Protein DNA Advanced_DNA
Genetic context
YkrL context.gif
This image was kindly provided by SubtiList
Expression at a glance   PubMed
YkrL expression.png















Categories containing this gene/protein

membrane proteins, proteolysis

This gene is a member of the following regulons

Rok regulon, YkrK regulon,

The gene

Basic information

  • Locus tag: BSU13490

Phenotypes of a mutant

  • increased sensitivity to membrane protein overproduction PubMed
  • increased sensitivity to dissipation of the membrane potential by the addition of valinomycin PubMed

Database entries

  • DBTBS entry: no entry
  • SubtiList entry: [1]

Additional information

The protein

Basic information/ Evolution

  • Catalyzed reaction/ biological activity:
  • Protein family: peptidase M48B family (according to Swiss-Prot)
  • Paralogous protein(s):

Extended information on the protein

  • Kinetic information:
  • Domains:
  • Modification:
  • Cofactor(s):
  • Effectors of protein activity:

Database entries

  • Structure:
  • KEGG entry: [2]
  • E.C. number:

Additional information

Expression and regulation

  • Sigma factor:
  • Regulation:
    • induced by membrane protein overproduction (YkrK) PubMed
    • induced by dissipation of membrane potential (ΔΨ) by addition of a sublethal concentration of valinomycin PubMed
    • expression is increased due to mutations in resE, resB, sdhC, and sdhA PubMed
    • induced by salt stress PubMed
  • Regulatory mechanism:
  • Additional information:

Biological materials

  • Mutant:
  • Expression vector:
  • lacZ fusion:
  • GFP fusion:
  • two-hybrid system:
  • Antibody:

Labs working on this gene/protein

Your additional remarks

References

Ta-Hui Lin, Shih-Chien Huang, Gwo-Chyuan Shaw
Reexamining transcriptional regulation of the Bacillus subtilis htpX gene and the ykrK gene, encoding a novel type of transcriptional regulator, and redefining the YkrK operator.
J Bacteriol: 2012, 194(24);6758-65
[PubMed:23042994] [WorldCat.org] [DOI] (I p)

Bogumiła C Marciniak, Hein Trip, Patricia J van-der Veek, Oscar P Kuipers
Comparative transcriptional analysis of Bacillus subtilis cells overproducing either secreted proteins, lipoproteins or membrane proteins.
Microb Cell Fact: 2012, 11;66
[PubMed:22624725] [WorldCat.org] [DOI] (I e)

Bogumila C Marciniak, Hein Trip, Fabrizia Fusetti, Oscar P Kuipers
Regulation of ykrL (htpX) by Rok and YkrK, a novel type of regulator in Bacillus subtilis.
J Bacteriol: 2012, 194(11);2837-45
[PubMed:22447908] [WorldCat.org] [DOI] (I p)

Pierre Nicolas, Ulrike Mäder, Etienne Dervyn, Tatiana Rochat, Aurélie Leduc, Nathalie Pigeonneau, Elena Bidnenko, Elodie Marchadier, Mark Hoebeke, Stéphane Aymerich, Dörte Becher, Paola Bisicchia, Eric Botella, Olivier Delumeau, Geoff Doherty, Emma L Denham, Mark J Fogg, Vincent Fromion, Anne Goelzer, Annette Hansen, Elisabeth Härtig, Colin R Harwood, Georg Homuth, Hanne Jarmer, Matthieu Jules, Edda Klipp, Ludovic Le Chat, François Lecointe, Peter Lewis, Wolfram Liebermeister, Anika March, Ruben A T Mars, Priyanka Nannapaneni, David Noone, Susanne Pohl, Bernd Rinn, Frank Rügheimer, Praveen K Sappa, Franck Samson, Marc Schaffer, Benno Schwikowski, Leif Steil, Jörg Stülke, Thomas Wiegert, Kevin M Devine, Anthony J Wilkinson, Jan Maarten van Dijl, Michael Hecker, Uwe Völker, Philippe Bessières, Philippe Noirot
Condition-dependent transcriptome reveals high-level regulatory architecture in Bacillus subtilis.
Science: 2012, 335(6072);1103-6
[PubMed:22383849] [WorldCat.org] [DOI] (I p)