clpQ

168

two-component ATP-dependent protease

locus: BSU_16150

Molecular weight: 19.33 kDa

pI: 6.11

Protein length: 181 aa

Gene length: 546 bp

function: protein degradation

product: two-component ATP-dependent protease

essential: no

synonyms: clpQ, hslV, codW

List of homologs in different organisms, belongs to COG5405 (Galperin et al., 2021)

Gene

Coordinates: 1,688,114 → 1,688,659

Phenotypes of a mutant

• impaired in swarming motility [pubmed|29629859]

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The protein

Protein family

• peptidase T1B family (single member, according to UniProt)

Structure

• [PDB|2Z3B]
• [AF|P39070]

[wiki|Localization]

• cytoplasm, forms small foci, usually positioned near the cell membrane, formation of foci depends on the presence of the cognate ATPase [protein|2A5A080273CB7698DFABB147F4143E90BBCA3B01|clpY] [Pubmed|18689473]

Expression and Regulation

Operons

• genes: [gene|B6062575849EA65BB7B44A9723676885799E2759|trmFO]-[gene|89746E312F20C81BE3CFDFCE7D99B01C375C198B|codV]-[gene|F6B748621B312E0B5A0A0782F3944C3E8112D7A8|clpQ]-[gene|2A5A080273CB7698DFABB147F4143E90BBCA3B01|clpY]-[gene|90ACE0DECA58D3C1A55E135120F7A0C1C920571C|codY]

  description: [Pubmed|22383849]

  regulation

  • repressed during growth in the presence of branched chain amino acids ([protein|90ACE0DECA58D3C1A55E135120F7A0C1C920571C|codY]) [Pubmed|11331605]

  regulatory mechanism

  • [protein|90ACE0DECA58D3C1A55E135120F7A0C1C920571C|codY]: repression, [Pubmed|11331605], in [regulon|protein:90ACE0DECA58D3C1A55E135120F7A0C1C920571C|codY regulon]

  sigma factors

  • [protein|360F48D576DE950DF79C1A2677B7A35A8D8CC30C|sigA]: sigma factor, [Pubmed|7783641], in [regulon|protein:360F48D576DE950DF79C1A2677B7A35A8D8CC30C|sigA regulon]

  additional information

  • the intracellular concentration of CodY is about 2.5 myM (according to [PubMed|20408793])

Biological materials

Mutant

• BKE16150 (Δ[gene|F6B748621B312E0B5A0A0782F3944C3E8112D7A8|clpQ]::erm  trpC2) available at [http://bgsc.org/getdetail.php?bgscid=BKE16150 BGSC],  [Pubmed|28189581], upstream reverse: _UP1_AAAAGATGACATAAAGGGCC,  downstream forward: _UP4_CTTGAATAGAAAGGACTTGA
• BKK16150 (Δ[gene|F6B748621B312E0B5A0A0782F3944C3E8112D7A8|clpQ]::kan  trpC2) available at [http://bgsc.org/getdetail.php?bgscid=BKK16150 BGSC],  [Pubmed|28189581], upstream reverse: _UP1_AAAAGATGACATAAAGGGCC,  downstream forward: _UP4_CTTGAATAGAAAGGACTTGA

References

Reviews

• 23479438,26639779,34410368

Original Publications

• 12805205,11179218,18689473,11331605,7783641,29629859,34293284