hprT
168
trigger enzyme, hypoxanthine phosphoribosyltransferase and part of a transcription activator
locus
BSU_00680
Molecular weight
20.10 kDa
pI
4.6
function
purine salvage and interconversion, control of [gene|4E7B9426CED372AA8A321A147116A3A589FBF20C|ftsH] expression
product
hypoxanthine phosphoribosyltransferase
essential
no
ec
2.4.2.8
synonyms
hprT
Outlinks
Genomic Context
Categories containing this gene/protein
List of homologs in different organisms, belongs to COG0634 (Galperin et al., 2021)
This gene is a member of the following regulons
Gene
Coordinates
76,344 → 76,886
Phenotypes of a mutant
essential [Pubmed|12682299], non-essential according to [Pubmed|28189581]
The protein
Catalyzed reaction/ biological activity
diphosphate + IMP --> 5-phospho-α-D-ribose 1-diphosphate + hypoxanthine (according to UniProt)
diphosphate + GMP --> 5-phospho-α-D-ribose 1-diphosphate + guanine (according to UniProt)
Protein family
[wiki|Purine/pyrimidine phosphoribosyltransferase family] (according to UniProt)
Structure
[PDB|6D9Q] (apo-protein) [pubmed|31552824]
[PDB|6D9R] (complex with the substrate PRPP) [pubmed|31552824]
[PDB|6D9S] (complex with ppGpp) [pubmed|31552824]
[AF|P37472]
Effectors of protein activity
inhibition of enzymatic activity by (p)ppGpp during the ´stringent response´, (p)ppGpp binds to the acive site of the enzyme and prevents the conversion of the apo-tetramer to the active dimer [Pubmed|31552824,22981860]
Kinetic information
KM (PRPP): 166 µM [pubmed|31552824]
KI (pppGpp): 1.7 µM [pubmed|31552824]
[wiki|Localization]
cytoplasm (according to UniProt)
Biological materials
Mutant
BKE00680 (Δ[gene|B7BFC170EEC88DEC6CF932F80805D3F2C0BD3738|hprT]::erm trpC2) available at [http://bgsc.org/getdetail.php?bgscid=BKE00680 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CATCATGATTTTGCTTGCCC, downstream forward: _UP4_TGATCGGCAGCCTGCTTCCG
BKK00680 (Δ[gene|B7BFC170EEC88DEC6CF932F80805D3F2C0BD3738|hprT]::kan trpC2) available at [http://bgsc.org/getdetail.php?bgscid=BKK00680 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CATCATGATTTTGCTTGCCC, downstream forward: _UP4_TGATCGGCAGCCTGCTTCCG
References
Reviews
Original Publications
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Time of last update: 2024-04-24 12:24:28
Author of last update: Jstuelk