hprT

168

trigger enzyme, hypoxanthine phosphoribosyltransferase and part of a transcription activator

locus: BSU_00680

Molecular weight: 20.10 kDa

pI: 4.6

Protein length: 180 aa

Gene length: 543 bp

function: purine salvage and interconversion, control of [gene|4E7B9426CED372AA8A321A147116A3A589FBF20C|ftsH] expression

product: hypoxanthine phosphoribosyltransferase

essential: no

ec: 2.4.2.8

synonyms: hprT

List of homologs in different organisms, belongs to COG0634 (Galperin et al., 2021)

Gene

Coordinates: 76,344 → 76,886

Phenotypes of a mutant

• essential [Pubmed|12682299], non-essential according to [Pubmed|28189581]

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The protein

Catalyzed reaction/ biological activity

• diphosphate + IMP --> 5-phospho-α-D-ribose 1-diphosphate + hypoxanthine (according to UniProt)
• diphosphate + GMP --> 5-phospho-α-D-ribose 1-diphosphate + guanine (according to UniProt)

Protein family

• [wiki|Purine/pyrimidine phosphoribosyltransferase family] (according to UniProt)

Structure

• [PDB|6D9Q] (apo-protein) [pubmed|31552824]
• [PDB|6D9R] (complex with the substrate PRPP) [pubmed|31552824]
• [PDB|6D9S] (complex with ppGpp) [pubmed|31552824]
• [AF|P37472]

Effectors of protein activity

• inhibition of enzymatic activity by (p)ppGpp during the ´stringent response´, (p)ppGpp binds to the acive site of the enzyme and prevents the conversion of the apo-tetramer to the active dimer [Pubmed|31552824,22981860]

Kinetic information

• K_M (PRPP):  166 µM [pubmed|31552824]
• K_I (pppGpp): 1.7 µM [pubmed|31552824]

[wiki|Localization]

• cytoplasm (according to UniProt)

Biological materials

Mutant

• BKE00680 (Δ[gene|B7BFC170EEC88DEC6CF932F80805D3F2C0BD3738|hprT]::erm  trpC2) available at [http://bgsc.org/getdetail.php?bgscid=BKE00680 BGSC],  [Pubmed|28189581], upstream reverse: _UP1_CATCATGATTTTGCTTGCCC,  downstream forward: _UP4_TGATCGGCAGCCTGCTTCCG
• BKK00680 (Δ[gene|B7BFC170EEC88DEC6CF932F80805D3F2C0BD3738|hprT]::kan  trpC2) available at [http://bgsc.org/getdetail.php?bgscid=BKK00680 BGSC],  [Pubmed|28189581], upstream reverse: _UP1_CATCATGATTTTGCTTGCCC,  downstream forward: _UP4_TGATCGGCAGCCTGCTTCCG

References

Reviews

• 28031352

Original Publications

• 18179421,22981860,24001521,6408059,28189581,31552824