Difference between revisions of "HprT"

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* '''Description:''' hypoxanthine phosphoribosyltransferase <br/><br/>
+
* '''Description:''' [[trigger enzyme]],hypoxanthine phosphoribosyltransferase and part of a transcription activator<br/><br/>
  
 
{| align="right" border="1" cellpadding="2"  
 
{| align="right" border="1" cellpadding="2"  
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|style="background:#ABCDEF;" align="center"| '''Product''' || hypoxanthine phosphoribosyltransferase
 
|style="background:#ABCDEF;" align="center"| '''Product''' || hypoxanthine phosphoribosyltransferase
 
|-
 
|-
|style="background:#ABCDEF;" align="center"|'''Function''' || purine salvage and interconversion
+
|style="background:#ABCDEF;" align="center"|'''Function''' || purine salvage and interconversion, control of ''[[ftsH]]'' expression
 
|-
 
|-
 
|colspan="2" style="background:#FAF8CC;" align="center"| '''Gene expression levels in [http://subtiwiki.uni-goettingen.de/apps/expression/ ''Subti''Express]''': [http://subtiwiki.uni-goettingen.de/apps/expression/expression.php?search=BSU00680 hprT]
 
|colspan="2" style="background:#FAF8CC;" align="center"| '''Gene expression levels in [http://subtiwiki.uni-goettingen.de/apps/expression/ ''Subti''Express]''': [http://subtiwiki.uni-goettingen.de/apps/expression/expression.php?search=BSU00680 hprT]
 +
|-
 +
|colspan="2" style="background:#FAF8CC;" align="center"| '''Interactions involving this protein in [http://cellpublisher.gobics.de/subtinteract/startpage/start/ ''Subt''Interact]''': [http://cellpublisher.gobics.de/subtinteract/interactionList/2/HprT HprT]
 
|-
 
|-
 
|colspan="2" style="background:#FAF8CC;" align="center"| '''Metabolic function and regulation of this protein in [[SubtiPathways|''Subti''Pathways]]: <br/>[http://subtiwiki.uni-goettingen.de/pathways/purines_salvage_pathways.html Purine salvage], [http://subtiwiki.uni-goettingen.de/pathways/purine_biosynthesis.html Purine synthesis], [http://subtiwiki.uni-goettingen.de/pathways/gene_regulation_nucleotides.html Nucleotides (regulation)]'''
 
|colspan="2" style="background:#FAF8CC;" align="center"| '''Metabolic function and regulation of this protein in [[SubtiPathways|''Subti''Pathways]]: <br/>[http://subtiwiki.uni-goettingen.de/pathways/purines_salvage_pathways.html Purine salvage], [http://subtiwiki.uni-goettingen.de/pathways/purine_biosynthesis.html Purine synthesis], [http://subtiwiki.uni-goettingen.de/pathways/gene_regulation_nucleotides.html Nucleotides (regulation)]'''
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= [[Categories]] containing this gene/protein =
 
= [[Categories]] containing this gene/protein =
 
{{SubtiWiki category|[[biosynthesis/ acquisition of nucleotides]]}},
 
{{SubtiWiki category|[[biosynthesis/ acquisition of nucleotides]]}},
 +
{{SubtiWiki category|[[transcription factors and their control]]}},
 
{{SubtiWiki category|[[cell envelope stress proteins (controlled by SigM, V, W, X, Y)]]}},
 
{{SubtiWiki category|[[cell envelope stress proteins (controlled by SigM, V, W, X, Y)]]}},
 
{{SubtiWiki category|[[essential genes]]}}
 
{{SubtiWiki category|[[essential genes]]}}
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= This gene is a member of the following [[regulons]] =
 
= This gene is a member of the following [[regulons]] =
 
{{SubtiWiki regulon|[[SigM regulon]]}}
 
{{SubtiWiki regulon|[[SigM regulon]]}}
 +
 +
= The [[TilS-HprT regulon]] =
 +
* ''[[ftsH]]''
  
 
=The gene=
 
=The gene=
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* '''[[SubtInteract|Interactions]]:'''
 
* '''[[SubtInteract|Interactions]]:'''
 +
** [[TilS]]-[[HprT]], the complex activates transcription of ''[[ftsH]]'' {{PubMed|24001521}}
  
 
* '''[[Localization]]:''' cytoplasm (according to Swiss-Prot)
 
* '''[[Localization]]:''' cytoplasm (according to Swiss-Prot)

Revision as of 11:58, 11 September 2013

  • Description: trigger enzyme,hypoxanthine phosphoribosyltransferase and part of a transcription activator

Gene name hprT
Synonyms
Essential yes PubMed
Product hypoxanthine phosphoribosyltransferase
Function purine salvage and interconversion, control of ftsH expression
Gene expression levels in SubtiExpress: hprT
Interactions involving this protein in SubtInteract: HprT
Metabolic function and regulation of this protein in SubtiPathways:
Purine salvage, Purine synthesis, Nucleotides (regulation)
MW, pI 20 kDa, 4.603
Gene length, protein length 540 bp, 180 aa
Immediate neighbours tilS, ftsH
Sequences Protein DNA DNA_with_flanks
Genetic context
HprT context.gif
This image was kindly provided by SubtiList
Expression at a glance   PubMed
HprT expression.png















Categories containing this gene/protein

biosynthesis/ acquisition of nucleotides, transcription factors and their control, cell envelope stress proteins (controlled by SigM, V, W, X, Y), essential genes

This gene is a member of the following regulons

SigM regulon

The TilS-HprT regulon

The gene

Basic information

  • Locus tag: BSU00680

Phenotypes of a mutant

essential PubMed

Database entries

  • DBTBS entry: no entry
  • SubtiList entry: [1]

Additional information

The protein

Basic information/ Evolution

  • Catalyzed reaction/ biological activity: IMP + diphosphate = hypoxanthine + 5-phospho-alpha-D-ribose 1-diphosphate (according to Swiss-Prot)
  • Protein family: purine/pyrimidine phosphoribosyltransferase family (according to Swiss-Prot)
  • Paralogous protein(s):

Extended information on the protein

  • Kinetic information:
  • Domains:
  • Modification:
  • Cofactor(s):
  • Effectors of protein activity:
    • inhibition of enzymatic activity by (p)ppGpp during the ´stringent response´PubMed

Database entries

  • Structure: 1J7J (from Salmonella typhimurium, 51% identity, 73% similarity)
  • KEGG entry: [2]

Additional information

Expression and regulation

  • Regulation:
  • Regulatory mechanism:
  • Additional information:

Biological materials

  • Mutant:
  • Expression vector:
  • lacZ fusion:
  • GFP fusion:
  • two-hybrid system:
  • Antibody:

Labs working on this gene/protein

Your additional remarks

References

Ta-Hui Lin, Yi-Nei Hu, Gwo-Chyuan Shaw
Two enzymes, TilS and HprT, can form a complex to function as a transcriptional activator for the cell division protease gene ftsH in Bacillus subtilis.
J Biochem: 2014, 155(1);5-16
[PubMed:24001521] [WorldCat.org] [DOI] (I p)

Allison Kriel, Alycia N Bittner, Sok Ho Kim, Kuanqing Liu, Ashley K Tehranchi, Winnie Y Zou, Samantha Rendon, Rui Chen, Benjamin P Tu, Jue D Wang
Direct regulation of GTP homeostasis by (p)ppGpp: a critical component of viability and stress resistance.
Mol Cell: 2012, 48(2);231-41
[PubMed:22981860] [WorldCat.org] [DOI] (I p)

Warawan Eiamphungporn, John D Helmann
The Bacillus subtilis sigma(M) regulon and its contribution to cell envelope stress responses.
Mol Microbiol: 2008, 67(4);830-48
[PubMed:18179421] [WorldCat.org] [DOI] (P p)