Difference between revisions of "Hom"

From SubtiWiki
Jump to: navigation, search
Line 35: Line 35:
 
=== Basic information ===
 
=== Basic information ===
  
* '''Locus tag:'''
+
* '''Locus tag:''' BSU32260
  
 
===Phenotypes of a mutant ===
 
===Phenotypes of a mutant ===
Line 80: Line 80:
 
* '''Swiss prot entry:''' [http://www.uniprot.org/uniprot/P19582 P19582]
 
* '''Swiss prot entry:''' [http://www.uniprot.org/uniprot/P19582 P19582]
  
* '''KEGG entry:''' [http://www.genome.jp/dbget-bin/www_bget?bsu+BSU32260 BSU32260]
+
* '''KEGG entry:''' [http://www.genome.jp/dbget-bin/www_bget?bsu+BSU32260]
  
 
* '''E.C. number:''' [http://www.expasy.org/enzyme/1.1.1.3 1.1.1.3]
 
* '''E.C. number:''' [http://www.expasy.org/enzyme/1.1.1.3 1.1.1.3]

Revision as of 13:52, 3 June 2009

  • Description: homoserine dehydrogenase (NADPH)

Gene name hom
Synonyms
Essential no
Product homoserine dehydrogenase (NADPH)
Function biosynthesis of methionine and threonine
MW, pI 47 kDa, 4.9
Gene length, protein length 1299 bp, 433 aa
Immediate neighbours thrC, yutH
Get the DNA and protein sequences
(Barbe et al., 2009)
Genetic context
Hom context.gif
This image was kindly provided by SubtiList



The gene

Basic information

  • Locus tag: BSU32260

Phenotypes of a mutant

Database entries

  • DBTBS entry: no entry
  • SubtiList entry: [1]

Additional information

The protein

Basic information/ Evolution

  • Catalyzed reaction/ biological activity: L-homoserine + NAD(P)+ = L-aspartate 4-semialdehyde + NAD(P)H (according to Swiss-Prot)
  • Protein family: homoserine dehydrogenase family (according to Swiss-Prot)
  • Paralogous protein(s):

Extended information on the protein

  • Kinetic information:
  • Domains:
  • Modification:
  • Cofactor(s):
  • Effectors of protein activity: subject to feedback inhibition PubMed
  • Interactions:
  • Localization: membrane associated PubMed

Database entries

  • Structure:
  • KEGG entry: [2]

Additional information

Expression and regulation

  • Regulation: repressed by casamino acids PubMed
  • Regulatory mechanism:
  • Additional information: subject to feedback inhibition PubMed

Biological materials

  • Mutant:
  • Expression vector:
  • lacZ fusion:
  • GFP fusion:
  • two-hybrid system:
  • Antibody:

Labs working on this gene/protein

Your additional remarks

References

  1. Mäder et al. (2002) Transcriptome and Proteome Analysis of Bacillus subtilis Gene Expression Modulated by Amino Acid Availability. J. Bacteriol 184: 1844288-4295 PubMed
  2. Hahne et al. (2008) From complementarity to comprehensiveness - targeting the membrane proteome of growing Bacillus subtilis by divergent approaches. Proteomics 8: 4123-4136 PubMed
  3. Parsot C, Cohen GN. (1988) Cloning and nucleotide sequence of the Bacillus subtilis hom gene coding for homoserine dehydrogenase. Structural and evolutionary relationships with Escherichia coli aspartokinases-homoserine dehydrogenases I and II. J Biol Chem. Oct 15;263(29): 14654-60. PubMed
  4. Author1, Author2 & Author3 (year) Title Journal volume: page-page. PubMed