hom

168

homoserine dehydrogenase (NADPH)

locus: BSU_32260

Molecular weight: 47.33 kDa

pI: 4.9

Protein length: 433 aa

Gene length: 1302 bp

function: biosynthesis of methionine and threonine

product: homoserine dehydrogenase (NADPH)

essential: no

ec: 1.1.1.3

synonyms: hom

List of homologs in different organisms, belongs to COG0460 (Galperin et al., 2021)

Gene

Coordinates: 3,314,828 3,316,129

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The protein

Catalyzed reaction/ biological activity

• L-aspartate 4-semialdehyde + NAD(P)H+H⁺ --> L-homoserine + NAD(P)⁺ [pubmed|6786715]

Protein family

• Homoserine dehydrogenase family (single member, according to UniProt)

[wiki|Domains]

• [wiki|ACT domain] (aa 350-426) (according to UniProt)

[wiki|Cofactors]

• NADPH [pubmed|6786715]

Structure

• [PDB|2EJW] (from ''Thermus thermophilus hb8'', 37% identity, 57% similarity)
• [AF|P19582]

Additional information

• belongs to the 100 most abundant proteins [PubMed|15378759]

Biological materials

Mutant

• BKE32260 ([gene|11421D5BD3AB6C205DDA0864F878E2B04340033F|hom]::erm trpC2) available at [http://bgsc.org/getdetail.php?bgscid=BKE32260 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CAAAAAAACTCCACCTTTCT, downstream forward: _UP4_GTAGAAGGGAACGGTTGGAG
• BKK32260 ([gene|11421D5BD3AB6C205DDA0864F878E2B04340033F|hom]::kan trpC2) available at [http://bgsc.org/getdetail.php?bgscid=BKK32260 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CAAAAAAACTCCACCTTTCT, downstream forward: _UP4_GTAGAAGGGAACGGTTGGAG

lacZ fusion

• pBP312 (in [wiki|pAC5]), available in [wiki|Fabian Commichau]'s lab [Pubmed|27260660]

References

• 12107147,18763711,3139660,19258532,24163341,25777134,15378759,25755103,27260660,6786715,33046675