hmoB

168

heme monooxygenase

locus: BSU_10100

Molecular weight: 18.68 kDa

pI: 5.22

Protein length: 166 aa

Gene length: 501 bp

function: degradation of heme, acquisition of iron

product: heme monooxygenase

essential: no

ec: null

synonyms: hmoB, yixC, yhgC

List of homologs in different organisms, belongs to COG2329 (Galperin et al., 2021)

Gene

Coordinates: 1,083,229  1,083,729

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The protein

Catalyzed reaction/ biological activity

• binds hemin ''in vitro'' with ~1:1 stoichiometry and degrade hemin in the presence of an electron donor [Pubmed|21873409]
• heme b + 3 O₂ + 3 reduced [NADPH—hemoprotein reductase] --> biliverdin IXα + CO + Fe²⁺ + H⁺ + 3 H₂O + 3 oxidized [NADPH—hemoprotein reductase] (according to UniProt)

Protein family

• antibiotic biosynthesis monooxygenase family (with [protein|A4E1A1FA886980C863919DA011AC1EEDDBD74967|hmoA], according to UniProt)

[wiki|Domains]

• [wiki|ABM domain] (aa 66-153) (according to UniProt)

Structure

• [PDB|3TVZ] [Pubmed|22531134]
• [AF|P38049]

Expression and Regulation

Operons

• genes: [gene|4EE1809AC405170A78F6F8C8C02EEF5CC54DE076|hmoB]

  description: [Pubmed|8335642]

  regulation

  • constitutively expressed [Pubmed|21873409]

Biological materials

Mutant

• MGNA-B503 (yhgC::erm), available at the [https://shigen.nig.ac.jp/bsub/resource/strainGeneDisrupted/detail/1502 NBRP B. subtilis, Japan]
• BKE10100 ([gene|4EE1809AC405170A78F6F8C8C02EEF5CC54DE076|hmoB]::erm  trpC2) available at [http://bgsc.org/getdetail.php?bgscid=BKE10100 BGSC],  [Pubmed|28189581], upstream reverse: _UP1_CATATCAGCCACCTCCTGCT,  downstream forward: _UP4_TAGCTGCCGGCATGTCCGGC
• BKK10100 ([gene|4EE1809AC405170A78F6F8C8C02EEF5CC54DE076|hmoB]::kan  trpC2) available at [http://bgsc.org/getdetail.php?bgscid=BKK10100 BGSC],  [Pubmed|28189581], upstream reverse: _UP1_CATATCAGCCACCTCCTGCT,  downstream forward: _UP4_TAGCTGCCGGCATGTCCGGC

References

• 8335642,22531134,24582752,21873409,37198628