HisS

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  • Description: histidyl-tRNA synthetase

Gene name hisS
Synonyms
Essential yes PubMed
Product histidyl-tRNA synthetase
Function translation
Gene expression levels in SubtiExpress: hisS
Metabolic function and regulation of this protein in SubtiPathways:
tRNA charging
MW, pI 47 kDa, 5.229
Gene length, protein length 1272 bp, 424 aa
Immediate neighbours aspS, yrzK
Sequences Protein DNA DNA_with_flanks
Genetic context
HisS context.gif
This image was kindly provided by SubtiList
Expression at a glance   PubMed
HisS expression.png




























Categories containing this gene/protein

translation, essential genes

This gene is a member of the following regulons

T-box

The gene

Basic information

  • Locus tag: BSU27560

Phenotypes of a mutant

essential PubMed

Database entries

  • DBTBS entry: no entry
  • SubtiList entry: [1]

Additional information

The protein

Basic information/ Evolution

  • Catalyzed reaction/ biological activity: ATP + L-histidine + tRNA(His) = AMP + diphosphate + L-histidyl-tRNA(His) (according to Swiss-Prot)
  • Protein family: class-II aminoacyl-tRNA synthetase family (according to Swiss-Prot)
  • Paralogous protein(s):

Extended information on the protein

  • Kinetic information:
  • Domains:
  • Modification:
  • Cofactor(s):
  • Effectors of protein activity:

Database entries

  • Structure: 1H4V (from Thermus thermophilus, 43% identity, 61% similarity) PubMed
  • KEGG entry: [2]

Additional information

Expression and regulation

  • Sigma factor:
  • Regulation:
    • induced by histidine or aspartate limitation (T-box) PubMed
  • Additional information:

Biological materials

  • Mutant:
  • Expression vector:
  • lacZ fusion:
  • GFP fusion:
  • two-hybrid system:
  • Antibody:

Labs working on this gene/protein

Your additional remarks

References

Ana Gutiérrez-Preciado, Tina M Henkin, Frank J Grundy, Charles Yanofsky, Enrique Merino
Biochemical features and functional implications of the RNA-based T-box regulatory mechanism.
Microbiol Mol Biol Rev: 2009, 73(1);36-61
[PubMed:19258532] [WorldCat.org] [DOI] (I p)