Difference between revisions of "HisS"

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(Extended information on the protein)
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* '''Effectors of protein activity:'''
 
* '''Effectors of protein activity:'''
  
* '''Interactions:'''
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* '''[[SubtInteract|Interactions]]:'''
  
* '''Localization:''' cytoplasm (according to Swiss-Prot)
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* '''[[Localization]]:'''
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** cytoplasm (according to Swiss-Prot)
  
 
=== Database entries ===
 
=== Database entries ===

Revision as of 20:30, 21 November 2011

  • Description: histidyl-tRNA synthetase

Gene name hisS
Synonyms
Essential yes PubMed
Product histidyl-tRNA synthetase
Function translation
Metabolic function and regulation of this protein in SubtiPathways:
tRNA charging
MW, pI 47 kDa, 5.229
Gene length, protein length 1272 bp, 424 aa
Immediate neighbours aspS, yrzK
Get the DNA and protein sequences
(Barbe et al., 2009)
Genetic context
HisS context.gif
This image was kindly provided by SubtiList







Categories containing this gene/protein

translation, essential genes

This gene is a member of the following regulons

T-box

The gene

Basic information

  • Locus tag: BSU27560

Phenotypes of a mutant

essential PubMed

Database entries

  • DBTBS entry: no entry
  • SubtiList entry: [1]

Additional information

The protein

Basic information/ Evolution

  • Catalyzed reaction/ biological activity: ATP + L-histidine + tRNA(His) = AMP + diphosphate + L-histidyl-tRNA(His) (according to Swiss-Prot)
  • Protein family: class-II aminoacyl-tRNA synthetase family (according to Swiss-Prot)
  • Paralogous protein(s):

Extended information on the protein

  • Kinetic information:
  • Domains:
  • Modification:
  • Cofactor(s):
  • Effectors of protein activity:

Database entries

  • Structure: 1H4V (from Thermus thermophilus, 43% identity, 61% similarity) PubMed
  • KEGG entry: [2]

Additional information

Expression and regulation

  • Regulation:
    • induced by histidine or aspartate limitation (T-box) PubMed
  • Additional information:

Biological materials

  • Mutant:
  • Expression vector:
  • lacZ fusion:
  • GFP fusion:
  • two-hybrid system:
  • Antibody:

Labs working on this gene/protein

Your additional remarks

References

Ana Gutiérrez-Preciado, Tina M Henkin, Frank J Grundy, Charles Yanofsky, Enrique Merino
Biochemical features and functional implications of the RNA-based T-box regulatory mechanism.
Microbiol Mol Biol Rev: 2009, 73(1);36-61
[PubMed:19258532] [WorldCat.org] [DOI] (I p)