hisI

168

phosphoribosyl-AMP cyclohydrolase / phosphoribosyl-ATP pyrophosphohydrolase

locus: BSU_34860

Molecular weight: 23.75 kDa

pI: 4.63

Protein length: 209 aa

Gene length: 630 bp

function: biosynthesis of histidine

product: phosphoribosyl-AMP cyclohydrolase /phosphoribosyl-ATP pyrophosphohydrolase

essential: no

ec: 3.5.4.19

synonyms: hisI, hisIE

List of homologs in different organisms, belongs to COG0140 (Galperin et al., 2021)

Gene

Coordinates: 3,582,936  3,583,565

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The protein

Catalyzed reaction/ biological activity

• 1-(5-phospho-β-D-ribosyl)-ATP + H₂O --> 1-(5-phospho-β-D-ribosyl)-5'-AMP + diphosphate + H⁺ (according to UniProt)
• 1-(5-phospho-β-D-ribosyl)-5'-AMP + H₂O --> 1-(5-phospho-β-D-ribosyl)-5-[(5-phospho-β-D-ribosylamino)methylideneamino]imidazole-4-carboxamide (according to UniProt)

[wiki|Domains]

• N-terminal domain (aa 1 ... 116): phosphoribosyl-AMP cyclohydrolase domain (according to UniProt) [pubmed|16042384]
• C-terminal domain (aa 117 ... 209): phosphoribosyl-ATP pyrophosphohydrolase domain (according to UniProt)

Structure

• [PDB|1ZPS] (the N-terminal domain, from Methanobacterium thermoautotrophicum, 49% identity)
• [PDB|1YVW] (the C-terminal domain, from B. cereus, 49% identity)
• [AF|O34912]

[wiki|Localization]

• cytoplasm (according to Swiss-Prot)

Biological materials

Mutant

• BKE34860 ([gene|5C84CA2A9E22173E144F519389F545F107A267D9|hisI]::erm  trpC2) available at [http://bgsc.org/getdetail.php?bgscid=BKE34860 BGSC],  [Pubmed|28189581], upstream reverse: _UP1_AAAACGCAGTTCATCTGCCT,  downstream forward: _UP4_TAAAGAGCCGGATGATACGG
• BKK34860 ([gene|5C84CA2A9E22173E144F519389F545F107A267D9|hisI]::kan  trpC2) available at [http://bgsc.org/getdetail.php?bgscid=BKK34860 BGSC],  [Pubmed|28189581], upstream reverse: _UP1_AAAACGCAGTTCATCTGCCT,  downstream forward: _UP4_TAAAGAGCCGGATGATACGG

References

• 12107147,27766092,16042384