hisI

hisI
168

phosphoribosyl-AMP cyclohydrolase / phosphoribosyl-ATP pyrophosphohydrolase

locus
BSU_34860
Molecular weight
23.75 kDa
pI
4.63
Protein length
Gene length
function
biosynthesis of histidine
product
phosphoribosyl-AMP cyclohydrolase /phosphoribosyl-ATP pyrophosphohydrolase
essential
no
ec
3.5.4.19
synonyms
hisI, hisIE

Genomic Context

Categories containing this gene/protein

List of homologs in different organisms, belongs to COG0140 (Galperin et al., 2021)

This gene is a member of the following regulons

Gene
Coordinates
3,582,936  3,583,565
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The protein
Catalyzed reaction/ biological activity
1-(5-phospho-β-D-ribosyl)-ATP + H2O --> 1-(5-phospho-β-D-ribosyl)-5'-AMP + diphosphate + H+ (according to UniProt)
1-(5-phospho-β-D-ribosyl)-5'-AMP + H2O --> 1-(5-phospho-β-D-ribosyl)-5-[(5-phospho-β-D-ribosylamino)methylideneamino]imidazole-4-carboxamide (according to UniProt)
[wiki|Domains]
N-terminal domain (aa 1 ... 116): phosphoribosyl-AMP cyclohydrolase domain (according to UniProt) [pubmed|16042384]
C-terminal domain (aa 117 ... 209): phosphoribosyl-ATP pyrophosphohydrolase domain (according to UniProt)
Structure
[PDB|1ZPS] (the N-terminal domain, from Methanobacterium thermoautotrophicum, 49% identity)
[PDB|1YVW] (the C-terminal domain, from B. cereus, 49% identity)
[AF|O34912]
[wiki|Localization]
cytoplasm (according to Swiss-Prot)
Expression and Regulation
Operons
genes
[gene|E8FD92F48BE16F87D7481262F4E49003A78DE78F|hisZ]-[gene|183C8B4F28E601D670B3D997D333AF2B5C4A4759|hisG]-[gene|03274B80B2DFE0A2939981B0E0CB8C34A9B0B102|hisD]-[gene|88ED20DFEBD5D7B985F99D4494142FBA699A1E91|hisB]-[gene|DEB3122A3EA36545759FAA6F86F71F971CA5F011|hisH]-[gene|148F0C7983250F50676D234DB713E532AA55DDBF|hisA]-[gene|C22C15754859B2510E52622E6A5DFAF97A7AD4E5|hisF]-[gene|5C84CA2A9E22173E144F519389F545F107A267D9|hisI]
description
[Pubmed|12107147]
regulation
expression during spore [wiki|germination] is strongly reduced under conditions of osmotic stress [Pubmed|27766092]
expression depends on functional [protein|F4097349A563503468A2A14F062AEAC532C7917A|rnpM] [pubmed|30355672]
regulatory mechanism
[protein|F4097349A563503468A2A14F062AEAC532C7917A|rnpM]: unknown, [pubmed|30355672], in [regulon|protein:F4097349A563503468A2A14F062AEAC532C7917A|rnpM regulon]
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[gene|E8FD92F48BE16F87D7481262F4E49003A78DE78F|hisZ]→[gene|5C84CA2A9E22173E144F519389F545F107A267D9|hisI]

2024-03-26 19:01:51

Jstuelk

75

896e17f5309df81d9427f38fec175515ad428723

B6AAF7601668444EDDC2FE084A87885740B98ACD

Biological materials
Mutant
BKE34860 ([gene|5C84CA2A9E22173E144F519389F545F107A267D9|hisI]::erm  trpC2) available at [http://bgsc.org/getdetail.php?bgscid=BKE34860 BGSC],  [Pubmed|28189581], upstream reverse: _UP1_AAAACGCAGTTCATCTGCCT,  downstream forward: _UP4_TAAAGAGCCGGATGATACGG
BKK34860 ([gene|5C84CA2A9E22173E144F519389F545F107A267D9|hisI]::kan  trpC2) available at [http://bgsc.org/getdetail.php?bgscid=BKK34860 BGSC],  [Pubmed|28189581], upstream reverse: _UP1_AAAACGCAGTTCATCTGCCT,  downstream forward: _UP4_TAAAGAGCCGGATGATACGG
References
12107147,27766092,16042384

5C84CA2A9E22173E144F519389F545F107A267D9

Page visits: 2930

Time of last update: 2024-03-28 06:12:34

Author of last update: Melvin.boenninger