hisH

hisH
168

imidazole glycerol phosphate synthase (glutaminase subunit)

locus
BSU_34890
Molecular weight
23.16 kDa
pI
5.72
Protein length
Gene length
function
biosynthesis of histidine
product
imidazole glycerol phosphate synthase (glutaminase subunit)
essential
no
synonyms
hisH

Genomic Context

Categories containing this gene/protein

List of homologs in different organisms, belongs to COG0118 (Galperin et al., 2021)

This gene is a member of the following regulons

Gene
Coordinates
3,585,051 → 3,585,689
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The protein
Catalyzed reaction/ biological activity
5-[(5-phospho-1-deoxy-D-ribulos-1-ylimino)methylamino]-1-(5-phospho-β-D-ribosyl)imidazole-4-carboxamide + L-glutamine --> 5-amino-1-(5-phospho-β-D-ribosyl)imidazole-4-carboxamide + D-erythro-1-(imidazol-4-yl)glycerol 3-phosphate + H+ + L-glutamate (according to UniProt)
H2O + L-glutamine --> L-glutamate + NH4+ (according to UniProt)
[wiki|Domains]
[wiki|Glutamine amidotransferase type-1 domain] (aa 1-211) (according to UniProt)
Structure
[PDB|1KA9] (the [protein|C22C15754859B2510E52622E6A5DFAF97A7AD4E5|hisF]-[protein|DEB3122A3EA36545759FAA6F86F71F971CA5F011|hisH] complex from ''Thermus thermophilus'', 39% identity) [Pubmed|12417026]
[AF|O34565]
[wiki|Localization]
cytoplasm (according to Swiss-Prot)
Expression and Regulation
Operons
genes
[gene|E8FD92F48BE16F87D7481262F4E49003A78DE78F|hisZ]-[gene|183C8B4F28E601D670B3D997D333AF2B5C4A4759|hisG]-[gene|03274B80B2DFE0A2939981B0E0CB8C34A9B0B102|hisD]-[gene|88ED20DFEBD5D7B985F99D4494142FBA699A1E91|hisB]-[gene|DEB3122A3EA36545759FAA6F86F71F971CA5F011|hisH]-[gene|148F0C7983250F50676D234DB713E532AA55DDBF|hisA]-[gene|C22C15754859B2510E52622E6A5DFAF97A7AD4E5|hisF]-[gene|5C84CA2A9E22173E144F519389F545F107A267D9|hisI]
description
[Pubmed|12107147]
regulation
expression during spore [wiki|germination] is strongly reduced under conditions of osmotic stress [Pubmed|27766092]
expression depends on functional [protein|F4097349A563503468A2A14F062AEAC532C7917A|rnpM] [pubmed|30355672]
regulatory mechanism
[protein|F4097349A563503468A2A14F062AEAC532C7917A|rnpM]: unknown, [pubmed|30355672], in [regulon|protein:F4097349A563503468A2A14F062AEAC532C7917A|rnpM regulon]
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[gene|E8FD92F48BE16F87D7481262F4E49003A78DE78F|hisZ]→[gene|5C84CA2A9E22173E144F519389F545F107A267D9|hisI]

2024-03-26 19:01:51

Jstuelk

75

896e17f5309df81d9427f38fec175515ad428723

B6AAF7601668444EDDC2FE084A87885740B98ACD

Biological materials
Mutant
BKE34890 (Δ[gene|DEB3122A3EA36545759FAA6F86F71F971CA5F011|hisH]::erm  trpC2) available at [http://bgsc.org/getdetail.php?bgscid=BKE34890 BGSC],  [Pubmed|28189581], upstream reverse: _UP1_TCCGTAATCTATCACGCCGA,  downstream forward: _UP4_GCAGCAGAACAGAAGGTGAA
BKK34890 (Δ[gene|DEB3122A3EA36545759FAA6F86F71F971CA5F011|hisH]::kan  trpC2) available at [http://bgsc.org/getdetail.php?bgscid=BKK34890 BGSC],  [Pubmed|28189581], upstream reverse: _UP1_TCCGTAATCTATCACGCCGA,  downstream forward: _UP4_GCAGCAGAACAGAAGGTGAA
References
Reviews
12859215
Original Publications
3106153,12107147,12417026,27766092

DEB3122A3EA36545759FAA6F86F71F971CA5F011

Page visits: 1705

Time of last update: 2024-03-28 14:01:34

Author of last update: Melvin.boenninger