hisF

168

imidazole glycerol phosphate synthase (synthase subunit)

locus: BSU_34870

Molecular weight: 27.14 kDa

pI: 5.24

Protein length: 252 aa

Gene length: 759 bp

function: biosynthesis of histidine

product: imidazole glycerol phosphate synthase (synthase subunit)

essential: no

synonyms: hisF

List of homologs in different organisms, belongs to COG0107 (Galperin et al., 2021)

Gene

Coordinates: 3,583,562 3,584,320

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The protein

Catalyzed reaction/ biological activity

• 5-[(5-phospho-1-deoxy-D-ribulos-1-ylimino)methylamino]-1-(5-phospho--D-ribosyl)imidazole-4-carboxamide + L-glutamine --> 5-amino-1-(5-phospho--D-ribosyl)imidazole-4-carboxamide + D-erythro-1-(imidazol-4-yl)glycerol 3-phosphate + H⁺ + L-glutamate (according to UniProt)

Protein family

• HisA/HisF family (with [protein|148F0C7983250F50676D234DB713E532AA55DDBF|hisA], according to UniProt)

Structure

• [PDB|1KA9] (the [protein|C22C15754859B2510E52622E6A5DFAF97A7AD4E5|hisF]-[protein|DEB3122A3EA36545759FAA6F86F71F971CA5F011|hisH] complex from ''Thermus thermophilus'', 59% identity) [Pubmed|12417026]
• [AF|O34727]

Paralogous protein(s)

• [protein|148F0C7983250F50676D234DB713E532AA55DDBF|hisA]

[wiki|Localization]

• cytoplasm (according to Swiss-Prot)

Additional information

• belongs to the 100 most abundant proteins [PubMed|15378759]

Biological materials

Mutant

• BKE34870 ([gene|C22C15754859B2510E52622E6A5DFAF97A7AD4E5|hisF]::erm trpC2) available at [http://bgsc.org/getdetail.php?bgscid=BKE34870 BGSC], [Pubmed|28189581], upstream reverse: _UP1_GCATGGGATAATCCGTTTTG, downstream forward: _UP4_AAAGAGTACGGGGTGAATGT
• BKK34870 ([gene|C22C15754859B2510E52622E6A5DFAF97A7AD4E5|hisF]::kan trpC2) available at [http://bgsc.org/getdetail.php?bgscid=BKK34870 BGSC], [Pubmed|28189581], upstream reverse: _UP1_GCATGGGATAATCCGTTTTG, downstream forward: _UP4_AAAGAGTACGGGGTGAATGT

References

Reviews

• 12859215

Original Publications

• 12107147,15378759,12417026,27766092