HisB

• Description: imidazoleglycerol-phosphate dehydratase
  
  

Gene name	hisB
Synonyms
Essential	no
Product	imidazoleglycerol-phosphate dehydratase
Function	biosynthesis of histidine
Metabolic function and regulation of this protein in SubtiPathways: His
MW, pI	21 kDa, 5.598
Gene length, protein length	582 bp, 194 aa
Immediate neighbours	hisH, hisD
Get the DNA and protein sequences (Barbe et al., 2009)
Genetic context This image was kindly provided by SubtiList

Categories containing this gene/protein

biosynthesis/ acquisition of amino acids

The gene

Basic information

• Locus tag: BSU34900

Database entries

• DBTBS entry: no entry

• SubtiList entry: [1]

The protein

Basic information/ Evolution

• Catalyzed reaction/ biological activity: D-erythro-1-(imidazol-4-yl)glycerol 3-phosphate = 3-(imidazol-4-yl)-2-oxopropyl phosphate + H₂O (according to Swiss-Prot)

• Protein family: imidazoleglycerol-phosphate dehydratase family (according to Swiss-Prot)

• Paralogous protein(s):

Extended information on the protein

• Kinetic information:

• Domains:

• Modification:

• Cofactor(s):

• Effectors of protein activity:

• Interactions:

• Localization:
  • cytoplasm (according to Swiss-Prot)

Database entries

• Structure: 2AE8 (from Staphylococcus aureus subsp. aureus n315 mutant, 48% identity, 65% similarity)

• UniProt: O34683

• KEGG entry: [2]

• E.C. number: 4.2.1.19

Expression and regulation

• Operon: hisZ-hisG-hisD-hisB-hisH-hisA-hisF-hisI PubMed

• Sigma factor:

• Regulation: repressed by casamino acids PubMed

• Regulatory mechanism:

• Additional information:

Biological materials

• Mutant:

• Expression vector:

• lacZ fusion:

• GFP fusion:

• two-hybrid system:

• Antibody:

References

Ulrike Mäder, Georg Homuth, Christian Scharf, Knut Büttner, Rüdiger Bode, Michael Hecker
Transcriptome and proteome analysis of Bacillus subtilis gene expression modulated by amino acid availability.
J Bacteriol: 2002, 184(15);4288-95
[PubMed:12107147] [WorldCat.org] [DOI] (P p)