HisA

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  • Description: phosphoribosylformimino-5-aminoimidazole carboxamide ribotide isomerase, homologue to HisF

Gene name hisA
Synonyms
Essential no
Product phosphoribosylformimino-5-aminoimidazole
carboxamide ribotide isomerase, homologue to HisF
Function biosynthesis of histidine
Gene expression levels in SubtiExpress: hisA
Metabolic function and regulation of this protein in SubtiPathways:
hisA
MW, pI 26 kDa, 4.842
Gene length, protein length 735 bp, 245 aa
Immediate neighbours hisF, hisH
Sequences Protein DNA DNA_with_flanks
Genetic context
HisA context.gif
This image was kindly provided by SubtiList
Expression at a glance   PubMed
HisA expression.png




























Categories containing this gene/protein

biosynthesis/ acquisition of amino acids

This gene is a member of the following regulons

The gene

Basic information

  • Locus tag: BSU34880

Phenotypes of a mutant

Database entries

  • DBTBS entry: no entry
  • SubtiList entry: [1]

Additional information

The protein

Basic information/ Evolution

  • Catalyzed reaction/ biological activity: 1-(5-phosphoribosyl)-5-((5-phosphoribosylamino)methylideneamino)imidazole-4-carboxamide = 5-((5-phospho-1-deoxyribulos-1-ylamino)methylideneamino)-1-(5-phosphoribosyl)imidazole-4-carboxamide (according to Swiss-Prot)
  • Protein family: hisA/hisF family (according to Swiss-Prot)
  • Paralogous protein(s): HisF

Extended information on the protein

  • Kinetic information:
  • Domains:
  • Modification:
  • Cofactor(s):
  • Effectors of protein activity:

Database entries

  • Structure: 2VEP (from Streptomyces coelicolor, 41% identity, 58% similarity) PubMed
  • KEGG entry: [2]

Additional information

Expression and regulation

  • Sigma factor:
  • Regulation: repressed by casamino acids PubMed
  • Regulatory mechanism:
  • Additional information:

Biological materials

  • Mutant:
  • Expression vector:
  • lacZ fusion:
  • GFP fusion:
  • two-hybrid system:
  • Antibody:

Labs working on this gene/protein

Your additional remarks

References

Ulrike Mäder, Georg Homuth, Christian Scharf, Knut Büttner, Rüdiger Bode, Michael Hecker
Transcriptome and proteome analysis of Bacillus subtilis gene expression modulated by amino acid availability.
J Bacteriol: 2002, 184(15);4288-95
[PubMed:12107147] [WorldCat.org] [DOI] (P p)