HemY

Gene name	hemY
Synonyms	hemG
Essential	no
Product	protoporphyrinogen IX oxidase
Function	heme biosynthesis
Gene expression levels in SubtiExpress: hemY
MW, pI	51 kDa, 8.042
Gene length, protein length	1410 bp, 470 aa
Immediate neighbours	hemH, yhgD
Get the DNA and protein sequences (Barbe et al., 2009)
Genetic context This image was kindly provided by SubtiList
Expression at a glance   PubMed

Categories containing this gene/protein

biosynthesis of cofactors

The gene

Basic information

• Locus tag: BSU10140

Expression

Database entries

• DBTBS entry: [1]

• SubtiList entry: [2]

The protein

Basic information/ Evolution

• Catalyzed reaction/ biological activity: Protoporphyrinogen-IX + 3 O₂ = protoporphyrin-IX + 3 H₂O₂ (according to Swiss-Prot)

• Protein family: protoporphyrinogen oxidase family (according to Swiss-Prot)

• Paralogous protein(s):

Extended information on the protein

• Kinetic information:

• Domains:

• Modification:

• Cofactor(s):

• Effectors of protein activity:

• Interactions:

• Localization:
  • cytoplasm (according to Swiss-Prot)

Database entries

• Structure: 3I6D

• UniProt: P32397

• KEGG entry: [3]

• E.C. number: 1.3.3.4

Expression and regulation

• Operon: hemE-hemH-hemY PubMed

• Expression browser: hemY PubMed

• Sigma factor:

• Regulation:

• Regulatory mechanism:

• Additional information:

Biological materials

• Mutant:

• Expression vector:

• lacZ fusion:

• GFP fusion:

• two-hybrid system:

• Antibody:

References

Ge-Fei Hao, Yang Zuo, Sheng-Gang Yang, Guang-Fu Yang
Protoporphyrinogen oxidase inhibitor: an ideal target for herbicide discovery.
Chimia (Aarau): 2011, 65(12);961-9
[PubMed:22273380] [WorldCat.org] [DOI] (P p)

Xiaohong Qin, Lu Sun, Xin Wen, Xue Yang, Ying Tan, Hao Jin, Qiongyao Cao, Weihong Zhou, Zhen Xi, Yuequan Shen
Structural insight into unique properties of protoporphyrinogen oxidase from Bacillus subtilis.
J Struct Biol: 2010, 170(1);76-82
[PubMed:19944166] [WorldCat.org] [DOI] (I p)

Lu Sun, Xin Wen, Ying Tan, Heyang Li, Xing Yang, Yuefang Zhao, Baifan Wang, Qiongyao Cao, Congwei Niu, Zhen Xi
Site-directed mutagenesis and computational study of the Y366 active site in Bacillus subtilis protoporphyrinogen oxidase.
Amino Acids: 2009, 37(3);523-30
[PubMed:19266155] [WorldCat.org] [DOI] (I p)

M Hansson, M C Gustafsson, C G Kannangara, L Hederstedt
Isolated Bacillus subtilis HemY has coproporphyrinogen III to coproporphyrin III oxidase activity.
Biochim Biophys Acta: 1997, 1340(1);97-104
[PubMed:9217019] [WorldCat.org] [DOI] (P p)

M Hansson, L Hederstedt
Bacillus subtilis HemY is a peripheral membrane protein essential for protoheme IX synthesis which can oxidize coproporphyrinogen III and protoporphyrinogen IX.
J Bacteriol: 1994, 176(19);5962-70
[PubMed:7928957] [WorldCat.org] [DOI] (P p)

M Hansson, L Hederstedt
Cloning and characterization of the Bacillus subtilis hemEHY gene cluster, which encodes protoheme IX biosynthetic enzymes.
J Bacteriol: 1992, 174(24);8081-93
[PubMed:1459957] [WorldCat.org] [DOI] (P p)