Difference between revisions of "HemAT"

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===Phenotypes of a mutant ===
 
===Phenotypes of a mutant ===
 +
* not essential for pellicle biofilm formation, but mutant is outcompeted by the wild-type strain when competed during pellicle formation {{PubMed|26122431}}
 +
* NCIB3610 ''hemAT'' mutant has higher fitness than ''[[hag]]'' mutant during pellicle formation {{PubMed|26122431}}
 +
* NCIB3610 ''hemAT''-''[[hag]]'' double mutant has similar fitness to single ''[[hag]]'' mutant during pellicle formation {{PubMed|26122431}}
  
 
=== Database entries ===
 
=== Database entries ===
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=The protein=
 
=The protein=
 +
* HemAT protein has a moderate oxygen affinity {{PubMed|11821422}}
  
 
=== Basic information/ Evolution ===
 
=== Basic information/ Evolution ===
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* '''BsubCyc:''' [http://bsubcyc.org/BSUB/NEW-IMAGE?type=NIL&object=BSU10380&redirect=T BSU10380]
 
* '''BsubCyc:''' [http://bsubcyc.org/BSUB/NEW-IMAGE?type=NIL&object=BSU10380&redirect=T BSU10380]
  
* '''Structure:''' [http://www.rcsb.org/pdb/explore.do?structureId=1OR4 1OR4] (in cyano-liganded form),  [http://www.rcsb.org/pdb/explore.do?structureId=1OR6 1OR6]
+
* '''Structure:''' [http://www.rcsb.org/pdb/explore.do?structureId=1OR4 1OR4] (in cyano-liganded form),  [http://www.rcsb.org/pdb/explore.do?structureId=1OR6 1OR6] {{PubMed|12962628}}
  
 
* '''UniProt:''' [http://www.uniprot.org/uniprot/O07621 O07621]
 
* '''UniProt:''' [http://www.uniprot.org/uniprot/O07621 O07621]
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=Biological materials =
 
=Biological materials =
 
* '''Mutant:'''
 
* '''Mutant:'''
 +
** TB239 (''hemAT''::''neo'' in 168) {{PubMed|26122431}}
 +
** TB241 (''hemAT''::''neo'' in NCIB3610) {{PubMed|26122431}}
 +
** TB244 ''amyE''::Phy-''sfgfp'' (''hemAT''::''neo'' in NCIB3610 with constitutive expressed ''sfgfp'') {{PubMed|26122431}}
 +
** TB243 ''amyE''::Phy-''mKATE2'' (''hemAT''::''neo'' in NCIB3610 with constitutive expressed ''mKATE2'') {{PubMed|26122431}}
  
 
* '''Expression vector:'''
 
* '''Expression vector:'''
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=Labs working on this gene/protein=
 
=Labs working on this gene/protein=
 +
* [[Akos T Kovacs]]
  
 
=Your additional remarks=
 
=Your additional remarks=
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<pubmed> 23928310 </pubmed>
 
<pubmed> 23928310 </pubmed>
 
== Original publications ==
 
== Original publications ==
<pubmed>10676961,16819829,11481493,15033535 21515776 23180473 22564695</pubmed>
+
<pubmed>26122431, 10676961,16819829,11481493,15033535 21515776 23180473 22564695</pubmed>
  
 
[[Category:Protein-coding genes]]
 
[[Category:Protein-coding genes]]

Revision as of 13:27, 2 July 2015

  • Description: soluble chemotaxis receptor, heme-containing O(2) sensor protein

Gene name hemAT
Synonyms yhfV
Essential no
Product haem-based aerotactic transducer
Function movement towards oxygen
Gene expression levels in SubtiExpress: hemAT
Interactions involving this protein in SubtInteract: HemAT
MW, pI 48 kDa, 5.441
Gene length, protein length 1296 bp, 432 aa
Immediate neighbours yhfU, yhfW
Sequences Protein DNA DNA_with_flanks
Genetic context
HemAT context.gif
This image was kindly provided by SubtiList
Expression at a glance   PubMed
HemAT expression.png















Categories containing this gene/protein

motility and chemotaxis

This gene is a member of the following regulons

SigD regulon

The gene

Basic information

  • Locus tag: BSU10380

Phenotypes of a mutant

  • not essential for pellicle biofilm formation, but mutant is outcompeted by the wild-type strain when competed during pellicle formation PubMed
  • NCIB3610 hemAT mutant has higher fitness than hag mutant during pellicle formation PubMed
  • NCIB3610 hemAT-hag double mutant has similar fitness to single hag mutant during pellicle formation PubMed

Database entries

  • DBTBS entry: [1]
  • SubtiList entry: [2]

Additional information

The protein

  • HemAT protein has a moderate oxygen affinity PubMed

Basic information/ Evolution

  • Catalyzed reaction/ biological activity:
  • Protein family:
  • Paralogous protein(s):

Extended information on the protein

  • Kinetic information:
  • Domains:
  • Modification:
  • Cofactor(s): heme
  • Effectors of protein activity:
  • Localization:
    • forms clusters at the cell poles PubMed
    • homogeneous cytoplsmic distribution in chain-forming cells during the logarithmic phase PubMed
    • polar foci in individual cells at later growth stages PubMed

Database entries

  • KEGG entry: [3]
  • E.C. number:

Additional information

Expression and regulation

  • Regulation:
  • Regulatory mechanism:
  • Additional information:
    • in minimal medium, HemAT is present with 19,000 +/- 3,900 molecules per cell PubMed
    • number of protein molecules per cell (minimal medium with glucose and ammonium): 1095 PubMed
    • number of protein molecules per cell (complex medium with amino acids, without glucose): 1803 PubMed
    • number of protein molecules per cell (minimal medium with glucose and ammonium, exponential phase): 3169 PubMed
    • number of protein molecules per cell (minimal medium with glucose and ammonium, early stationary phase after glucose exhaustion): 966 PubMed
    • number of protein molecules per cell (minimal medium with glucose and ammonium, late stationary phase after glucose exhaustion): 1236 PubMed

Biological materials

  • Mutant:
    • TB239 (hemAT::neo in 168) PubMed
    • TB241 (hemAT::neo in NCIB3610) PubMed
    • TB244 amyE::Phy-sfgfp (hemAT::neo in NCIB3610 with constitutive expressed sfgfp) PubMed
    • TB243 amyE::Phy-mKATE2 (hemAT::neo in NCIB3610 with constitutive expressed mKATE2) PubMed
  • Expression vector:
  • lacZ fusion:
  • GFP fusion:
  • two-hybrid system:
  • Antibody:

Labs working on this gene/protein

Your additional remarks

References

Reviews

Original publications