Difference between revisions of "HemAT"

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[[Category:Protein-coding genes]]
 
[[Category:Protein-coding genes]]

Revision as of 09:07, 9 May 2012

  • Description: soluble chemotaxis receptor, heme-containing O(2) sensor protein

Gene name hemAT
Synonyms yhfV
Essential no
Product haem-based aerotactic transducer
Function movement towards oxygen
MW, pI 48 kDa, 5.441
Gene length, protein length 1296 bp, 432 aa
Immediate neighbours yhfU, yhfW
Get the DNA and protein sequences
(Barbe et al., 2009)
Genetic context
HemAT context.gif
This image was kindly provided by SubtiList
Expression at a glance   PubMed
HemAT expression.png
























Categories containing this gene/protein

motility and chemotaxis

This gene is a member of the following regulons

SigD regulon

The gene

Basic information

  • Locus tag: BSU10380

Phenotypes of a mutant

Database entries

  • DBTBS entry: [1]
  • SubtiList entry: [2]

Additional information

The protein

Basic information/ Evolution

  • Catalyzed reaction/ biological activity:
  • Protein family:
  • Paralogous protein(s):

Extended information on the protein

  • Kinetic information:
  • Domains:
  • Modification:
  • Cofactor(s): heme
  • Effectors of protein activity:

Database entries

  • Structure: 1OR4 (in cyano-liganded form), 1OR6
  • KEGG entry: [3]
  • E.C. number:

Additional information

Expression and regulation

  • Regulation:
  • Regulatory mechanism:
  • Additional information:
    • in minimal medium, HemAT is present with 19,000 +/- 3,900 molecules per cell PubMed

Biological materials

  • Mutant:
  • Expression vector:
  • lacZ fusion:
  • GFP fusion:
  • two-hybrid system:
  • Antibody:

Labs working on this gene/protein

Your additional remarks

References

Additional publications: PubMed

Vincent J Cannistraro, George D Glekas, Christopher V Rao, George W Ordal
Cellular stoichiometry of the chemotaxis proteins in Bacillus subtilis.
J Bacteriol: 2011, 193(13);3220-7
[PubMed:21515776] [WorldCat.org] [DOI] (I p)

Hideaki Yoshimura, Shiro Yoshioka, Katsuaki Kobayashi, Takehiro Ohta, Takeshi Uchida, Minoru Kubo, Teizo Kitagawa, Shigetoshi Aono
Specific hydrogen-bonding networks responsible for selective O2 sensing of the oxygen sensor protein HemAT from Bacillus subtilis.
Biochemistry: 2006, 45(27);8301-7
[PubMed:16819829] [WorldCat.org] [DOI] (P p)

Masakuni Serizawa, Hiroki Yamamoto, Hirotake Yamaguchi, Yasutaro Fujita, Kazuo Kobayashi, Naotake Ogasawara, Junichi Sekiguchi
Systematic analysis of SigD-regulated genes in Bacillus subtilis by DNA microarray and Northern blotting analyses.
Gene: 2004, 329;125-36
[PubMed:15033535] [WorldCat.org] [DOI] (P p)

S Hou, T Freitas, R W Larsen, M Piatibratov, V Sivozhelezov, A Yamamoto, E A Meleshkevitch, M Zimmer, G W Ordal, M Alam
Globin-coupled sensors: a class of heme-containing sensors in Archaea and Bacteria.
Proc Natl Acad Sci U S A: 2001, 98(16);9353-8
[PubMed:11481493] [WorldCat.org] [DOI] (P p)

S Hou, R W Larsen, D Boudko, C W Riley, E Karatan, M Zimmer, G W Ordal, M Alam
Myoglobin-like aerotaxis transducers in Archaea and Bacteria.
Nature: 2000, 403(6769);540-4
[PubMed:10676961] [WorldCat.org] [DOI] (P p)