Difference between revisions of "Hbs"

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=== Database entries ===
 
=== Database entries ===
  
* '''Structure:''' [http://www.rcsb.org/pdb/explore.do?structureId=1EXE 1EXE] (phage SPO1 transcription factor, 45% identity)
+
* '''Structure:''' [http://www.rcsb.org/pdb/explore.do?structureId=1HUU 1HUU] (Geobacillus stearothermophilus),  [http://www.rcsb.org/pdb/explore.do?structureId=1EXE 1EXE] (phage SPO1 transcription factor, 45% identity)
  
 
* '''Swiss prot entry:''' [http://www.uniprot.org/uniprot/P42305 P42305]
 
* '''Swiss prot entry:''' [http://www.uniprot.org/uniprot/P42305 P42305]

Revision as of 22:21, 5 May 2009

  • Description: non-specific DNA-binding protein Hbsu

Gene name hbs
Synonyms dbpA
Essential yes PubMed
Product non-specific DNA-binding protein Hbsu
Function DNA packaging, function of the signal recognition complex
MW, pI 9 kDa, 9.501
Gene length, protein length 276 bp, 92 aa
Immediate neighbours mtrA, spoIVA
Get the DNA and protein sequences
(Barbe et al., 2009)
Genetic context
Hbs context.gif
This image was kindly provided by SubtiList



The gene

Basic information

  • Coordinates:

Phenotypes of a mutant

essential PubMed

Database entries

  • DBTBS entry: [1]
  • SubtiList entry: [2]

Additional information

The protein

Basic information/ Evolution

  • Catalyzed reaction/ biological activity:
  • Protein family:
  • Paralogous protein(s):

Extended information on the protein

  • Kinetic information:
  • Domains:
  • Modification: phosphorylation on Thr-4 PubMed
  • Cofactor(s):
  • Effectors of protein activity:
  • Interactions:
  • Localization: cytoplasm (according to Swiss-Prot), membrane associated PubMed

Database entries

  • Structure: 1HUU (Geobacillus stearothermophilus), 1EXE (phage SPO1 transcription factor, 45% identity)
  • KEGG entry: [3]
  • E.C. number:

Additional information

Expression and regulation

  • Operon:
  • Regulation:
  • Regulatory mechanism:
  • Additional information: mRNA is protected from degradation by RnjA by initiating ribosomes PubMed

Biological materials

  • Mutant:
  • Expression vector:
  • lacZ fusion:
  • GFP fusion:
  • two-hybrid system:
  • Antibody:

Labs working on this gene/protein

Ciaran Condon, IBPC, Paris, France Homepage

Your additional remarks

References

  1. Hahne et al. (2008) From complementarity to comprehensiveness - targeting the membrane proteome of growing Bacillus subtilis by divergent approaches. Proteomics 8: 4123-4136 PubMed
  2. Macek et al. (2007) The serine/ threonine/ tyrosine phosphoproteome of the model bacterium Bacillus subtilis. Mol. Cell. Proteomics 6: 697-707 PubMed
  3. Daou-Chabo R, Mathy N, Bénard L, Condon C. (2009) Ribosomes initiating translation of the hbs mRNA protect it from 5'-to-3' exoribonucleolytic degradation by RNase J1. Mol. Microbiol. 71: 1538-1550. PubMed
  4. Author1, Author2 & Author3 (year) Title Journal volume: page-page. PubMed