Difference between revisions of "Hag"

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(References)
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===Phenotypes of a mutant ===
 
===Phenotypes of a mutant ===
  
No swarming motility on B medium. [http://www.ncbi.nlm.nih.gov/sites/entrez/19202088 PubMed]
+
* no swarming motility on B medium. [http://www.ncbi.nlm.nih.gov/sites/entrez/19202088 PubMed]
 +
* not essential for pellicle biofilm formation, but mutant is outcompeted by the wild-type strain when competed during pellicle formation {{PubMed|26122431}}
 +
* few hours delay in pellicle development {{PubMed|26122431}}
 +
* mutant has increased fitness in planktonic culture when competed with the wild-type NCIB3610 strain {{PubMed|26122431}}
  
 
=== Database entries ===
 
=== Database entries ===
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** 1A783 ( ''hag''::''erm''), {{PubMed|2174860}}, available at [http://pasture.asc.ohio-state.edu/BGSC/getdetail.cfm?bgscid=1A783&Search=1A783 BGSC]
 
** 1A783 ( ''hag''::''erm''), {{PubMed|2174860}}, available at [http://pasture.asc.ohio-state.edu/BGSC/getdetail.cfm?bgscid=1A783&Search=1A783 BGSC]
 
** 1A842 ( ''hag''::''kan''), {{PubMed|14762011}}, available at [http://pasture.asc.ohio-state.edu/BGSC/getdetail.cfm?bgscid=1A842&Search=1A842 BGSC]
 
** 1A842 ( ''hag''::''kan''), {{PubMed|14762011}}, available at [http://pasture.asc.ohio-state.edu/BGSC/getdetail.cfm?bgscid=1A842&Search=1A842 BGSC]
 +
** DS1677 (marker-less  in NCIB3610) {{PubMed|18566286}}
 +
** TB191 ''amyE''::Phy-''sfgfp'' (marker-less in NCIB3610 with constitutive expressed ''sfgfp'') {{PubMed|26122431}}
 +
** TB207 ''amyE''::Phy-''mKATE2'' (marker-less in NCIB3610 with constitutive expressed ''mKATE2'') {{PubMed|26122431}}
 +
  
 
* '''Expression vector:'''
 
* '''Expression vector:'''
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=Labs working on this gene/protein=
 
=Labs working on this gene/protein=
 +
* [[Daniel Kearns]]
  
 
=Your additional remarks=
 
=Your additional remarks=
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<pubmed>22672726 25251856 </pubmed>
 
<pubmed>22672726 25251856 </pubmed>
 
==Original Publications==
 
==Original Publications==
<pubmed> 23144244 21602220 21856853 2498284,9648743,9096206,7708689,19202088, 18763711, 21895793 10809682 19118355 17555441 20534509 15378759</pubmed>
+
<pubmed> 18566286,26122431,23144244 21602220 21856853 2498284,9648743,9096206,7708689,19202088, 18763711, 21895793 10809682 19118355 17555441 20534509 15378759</pubmed>
 
[[Category:Protein-coding genes]]
 
[[Category:Protein-coding genes]]

Revision as of 13:08, 2 July 2015

  • Description: flagellin protein, about 20,000 subunits make up one flagellum

Gene name hag
Synonyms
Essential no
Product flagellin protein
Function motility and chemotaxis
Gene expression levels in SubtiExpress: hag
Interactions involving this protein in SubtInteract: Hag
Metabolic function and regulation of this protein in SubtiPathways:
Hag
MW, pI 32 kDa, 4.782
Gene length, protein length 912 bp, 304 aa
Immediate neighbours yvyC, csrA
Sequences Protein DNA DNA_with_flanks
Genetic context
Hag context.gif
This image was kindly provided by SubtiList
Expression at a glance   PubMed
Hag expression.png















Categories containing this gene/protein

motility and chemotaxis, most abundant proteins

This gene is a member of the following regulons

CodY regulon, CsrA regulon, ScoC regulon, SigD regulon

The gene

Basic information

  • Locus tag: BSU35360

Phenotypes of a mutant

  • no swarming motility on B medium. PubMed
  • not essential for pellicle biofilm formation, but mutant is outcompeted by the wild-type strain when competed during pellicle formation PubMed
  • few hours delay in pellicle development PubMed
  • mutant has increased fitness in planktonic culture when competed with the wild-type NCIB3610 strain PubMed

Database entries

  • DBTBS entry: [1]
  • SubtiList entry: [2]

Additional information

The protein

Basic information/ Evolution

  • Catalyzed reaction/ biological activity:
  • Protein family: bacterial flagellin family (according to Swiss-Prot)
  • Paralogous protein(s): YvzB (C-terminal domain of Hag)

Extended information on the protein

  • Kinetic information:
  • Modification:
  • Effectors of protein activity:

Database entries

  • Structure: 3A5X (from Salmonella typhimurium, 42% identity)
  • KEGG entry: [3]
  • E.C. number:

Additional information

Expression and regulation

  • Regulation:
    • repressed during growth in the presence of branched chain amino acids (CodY) PubMed
  • Additional information:
    • the hag gene is strongly overexpressed in a ymdB mutant (loss of bistable gene expression) PubMed
    • belongs to the 100 most abundant proteins PubMed
    • number of protein molecules per cell (minimal medium with glucose and ammonium): 150241 PubMed
    • number of protein molecules per cell (complex medium with amino acids, without glucose): 280953 PubMed
    • number of protein molecules per cell (minimal medium with glucose and ammonium, exponential phase): 9552 PubMed
    • number of protein molecules per cell (minimal medium with glucose and ammonium, early stationary phase after glucose exhaustion): 11147 PubMed
    • number of protein molecules per cell (minimal medium with glucose and ammonium, late stationary phase after glucose exhaustion): 24114 PubMed

Biological materials

  • Mutant: GP901 (aphA3), GP902 (tet) PubMed, both available in Jörg Stülke's lab
    • 1A915 ( hag::cat), PubMed, available at BGSC
    • 1A783 ( hag::erm), PubMed, available at BGSC
    • 1A842 ( hag::kan), PubMed, available at BGSC
    • DS1677 (marker-less in NCIB3610) PubMed
    • TB191 amyE::Phy-sfgfp (marker-less in NCIB3610 with constitutive expressed sfgfp) PubMed
    • TB207 amyE::Phy-mKATE2 (marker-less in NCIB3610 with constitutive expressed mKATE2) PubMed


  • Expression vector:
  • GFP fusion: BP494 (bglS:: (hag-promoter-cfp-aphA3)), BP496 (amyE:: (hag-promoter-iyfp-cat)), available in Jörg Stülke's lab
  • two-hybrid system:
  • Antibody:

Labs working on this gene/protein

Your additional remarks

References

Reviews

Original Publications