Difference between revisions of "GyrA"

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(Basic information/ Evolution)
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* '''Description:''' write here <br/><br/>
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* '''Description:''' DNA-Gyrase (subunit A) <br/><br/>
  
 
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|style="background:#ABCDEF;" align="center"| '''Essential''' || yes [http://www.ncbi.nlm.nih.gov/pubmed/12682299 PubMed]  
 
|style="background:#ABCDEF;" align="center"| '''Essential''' || yes [http://www.ncbi.nlm.nih.gov/pubmed/12682299 PubMed]  
 
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|-
|style="background:#ABCDEF;" align="center"| '''Product''' || DNA gyrase (subunit A) (EC 5.99.1.3)
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|style="background:#ABCDEF;" align="center"| '''Product''' || DNA-Gyrase (subunit A)
 
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|style="background:#ABCDEF;" align="center"|'''Function''' || initation of replication cycle and DNA elongation
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|style="background:#ABCDEF;" align="center"|'''Function''' || DNA superrcoiling, initation of replication cycle and DNA elongation
 
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|style="background:#ABCDEF;" align="center"| '''MW, pI''' || 91 kDa, 5.215   
 
|style="background:#ABCDEF;" align="center"| '''MW, pI''' || 91 kDa, 5.215   

Revision as of 16:36, 16 March 2009

  • Description: DNA-Gyrase (subunit A)

Gene name gyrA
Synonyms nalA
Essential yes PubMed
Product DNA-Gyrase (subunit A)
Function DNA superrcoiling, initation of replication cycle and DNA elongation
MW, pI 91 kDa, 5.215
Gene length, protein length 2463 bp, 821 aa
Immediate neighbours gyrB, rrnO-16S
Gene sequence (+200bp) Protein sequence
Genetic context
GyrA context.gif



The gene

Basic information

  • Coordinates:

Phenotypes of a mutant

essential PubMed

Database entries

  • DBTBS entry: [1]
  • SubtiList entry: [2]

Additional information

The protein

Basic information/ Evolution

  • Catalyzed reaction/ biological activity:
  • Protein family:
  • Paralogous protein(s): ParC

Extended information on the protein

  • Kinetic information:
  • Domains:
  • Modification:
  • Cofactor(s):
  • Effectors of protein activity:
  • Interactions: GyrA-GyrB
  • Localization: nucleoid-associated in actively growing cells PubMed

Database entries

  • Structure:
  • Swiss prot entry:
  • KEGG entry:
  • E.C. number:

Additional information

Expression and regulation

  • Regulation:
  • Regulatory mechanism:
  • Additional information: GyrA is subject to Clp-dependent proteolysis upon glucose starvation PubMed

Biological materials

  • Mutant:
  • Expression vector:
  • lacZ fusion:
  • GFP fusion:
  • two-hybrid system:
  • Antibody:

Labs working on this gene/protein

Your additional remarks

References

  1. Huang et al. (1998) Bipolar localization of Bacillus subtilis topoisomerase IV, an enzyme required for chromosome segregation. Proc. Natl. Acad. Sci. USA 95: 4652-4657. PubMed
  2. Gerth et al. (2008) Clp-dependent proteolysis down-regulates central metabolic pathways in glucose-starved Bacillus subtilis. J Bacteriol 190:321-331 PubMed
  3. Ogasawara et al. (1985) Structure and function of the region of the replication origin of the Bacillus subtilis chromosome. IV. Transcription of the oriC region and expression of DNA gyrase genes and other open reading frames.Nucl. Acids Res. 11: 2267-2279. PubMed