guaC

168

GMP reductase

locus: BSU_32130

Molecular weight: 35.66 kDa

pI: 6.08

Protein length: 326 aa

Gene length: 981 bp

function: purine salvage and interconversion

product: GMP reductase

essential: no

ec: 1.7.1.7

synonyms: guaC, yumD

List of homologs in different organisms, belongs to COG0516 (Galperin et al., 2021)

Gene

Coordinates: 3,303,042  3,304,022

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The protein

Catalyzed reaction/ biological activity

• Inosine 5'-phosphate + NH₃ + NADP⁺ --> guanosine 5'-phosphate + NADPH (according to UniProt)

Protein family

• IMPDH/GMPR family (together with [protein|AB3D18228DB6819B0C81BC7A8BB3408A4F75DC0C|guaB]) (according to UniProt)

Structure

• [PDB|1YPF] (from ''Bacillus anthracis'', 84% identity, 91% similarity)
• [AF|O05269]

Biological materials

Mutant

• MGNA-B573 (yumD::erm), available at the [https://shigen.nig.ac.jp/bsub/resource/strainGeneDisrupted/detail/1572 NBRP B. subtilis, Japan]
• BKE32130 ([gene|350A0C0D0ADB2A8E8DA9900E75A0D9914F18C665|guaC]::erm  trpC2) available at [http://bgsc.org/getdetail.php?bgscid=BKE32130 BGSC],  [Pubmed|28189581], upstream reverse: _UP1_CATGGTTTTCACTCCTAAAA,  downstream forward: _UP4_TAATCATAAAAAAACGCCAA
• BKK32130 ([gene|350A0C0D0ADB2A8E8DA9900E75A0D9914F18C665|guaC]::kan  trpC2) available at [http://bgsc.org/getdetail.php?bgscid=BKK32130 BGSC],  [Pubmed|28189581], upstream reverse: _UP1_CATGGTTTTCACTCCTAAAA,  downstream forward: _UP4_TAATCATAAAAAAACGCCAA

References

• 11591660,2536750,12618455,12850135,21815947