Difference between revisions of "GuaC"

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=== Database entries ===
 
=== Database entries ===
 +
* '''BsubCyc:''' [http://bsubcyc.org/BSUB/NEW-IMAGE?type=NIL&object=BSU32130&redirect=T BSU32130]
  
 
* '''DBTBS entry:''' [http://dbtbs.hgc.jp/COG/prom/guaC.html]
 
* '''DBTBS entry:''' [http://dbtbs.hgc.jp/COG/prom/guaC.html]
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=== Database entries ===
 
=== Database entries ===
 +
* '''BsubCyc:''' [http://bsubcyc.org/BSUB/NEW-IMAGE?type=NIL&object=BSU32130&redirect=T BSU32130]
  
 
* '''Structure:''' [http://www.rcsb.org/pdb/cgi/explore.cgi?pdbId=1YPF 1YPF] (from ''Bacillus anthracis'', 84% identity, 91% similarity)
 
* '''Structure:''' [http://www.rcsb.org/pdb/cgi/explore.cgi?pdbId=1YPF 1YPF] (from ''Bacillus anthracis'', 84% identity, 91% similarity)

Revision as of 14:40, 2 April 2014

  • Description: GMP reductase

Gene name guaC
Synonyms yumD
Essential no
Product GMP reductase
Function purine salvage and interconversion
Gene expression levels in SubtiExpress: guaC
Metabolic function and regulation of this protein in SubtiPathways:
guaC
MW, pI 35 kDa, 6.078
Gene length, protein length 978 bp, 326 aa
Immediate neighbours yuzG, paiB
Sequences Protein DNA DNA_with_flanks
Genetic context
GuaC context.gif
This image was kindly provided by SubtiList
Expression at a glance   PubMed
GuaC expression.png




























Categories containing this gene/protein

biosynthesis/ acquisition of nucleotides

This gene is a member of the following regulons

CodY regulon, PurR regulon

The gene

Basic information

  • Locus tag: BSU32130

Phenotypes of a mutant

Database entries

  • DBTBS entry: [1]
  • SubtiList entry: [2]

Additional information

The protein

Basic information/ Evolution

  • Catalyzed reaction/ biological activity: Inosine 5'-phosphate + NH3 + NADP+ = guanosine 5'-phosphate + NADPH (according to Swiss-Prot)
  • Protein family: GuaC type 2 subfamily (according to Swiss-Prot)
  • Paralogous protein(s):

Extended information on the protein

  • Kinetic information:
  • Domains:
  • Modification:
  • Cofactor(s):
  • Effectors of protein activity:

Database entries

  • Structure: 1YPF (from Bacillus anthracis, 84% identity, 91% similarity)
  • KEGG entry: [3]

Additional information

Expression and regulation

  • Sigma factor:
  • Regulation:
    • repressed in the presence of purine nucleotides (PurR) PubMed
    • activated during growth in the presence of branched chain amino acids (CodY) PubMed
    • expression activated by glucose (3.3 fold) PubMed
  • Regulatory mechanism:
    • CodY: transcription activation PubMed
    • PurR: transcription repression (molecular inducer: PRPP) PubMed
  • Additional information:
    • the mRNA is substantially stabilized upon depletion of RNase Y (the half-life of the mRNA increases from 3 to 20 min) PubMed

Biological materials

  • Mutant:
  • Expression vector:
  • lacZ fusion:
  • GFP fusion:
  • two-hybrid system:
  • Antibody:

Labs working on this gene/protein

Your additional remarks

References

Martin Lehnik-Habrink, Marc Schaffer, Ulrike Mäder, Christine Diethmaier, Christina Herzberg, Jörg Stülke
RNA processing in Bacillus subtilis: identification of targets of the essential RNase Y.
Mol Microbiol: 2011, 81(6);1459-73
[PubMed:21815947] [WorldCat.org] [DOI] (I p)

Hans-Matti Blencke, Georg Homuth, Holger Ludwig, Ulrike Mäder, Michael Hecker, Jörg Stülke
Transcriptional profiling of gene expression in response to glucose in Bacillus subtilis: regulation of the central metabolic pathways.
Metab Eng: 2003, 5(2);133-49
[PubMed:12850135] [WorldCat.org] [DOI] (P p)

Virginie Molle, Yoshiko Nakaura, Robert P Shivers, Hirotake Yamaguchi, Richard Losick, Yasutaro Fujita, Abraham L Sonenshein
Additional targets of the Bacillus subtilis global regulator CodY identified by chromatin immunoprecipitation and genome-wide transcript analysis.
J Bacteriol: 2003, 185(6);1911-22
[PubMed:12618455] [WorldCat.org] [DOI] (P p)

H H Saxild, K Brunstedt, K I Nielsen, H Jarmer, P Nygaard
Definition of the Bacillus subtilis PurR operator using genetic and bioinformatic tools and expansion of the PurR regulon with glyA, guaC, pbuG, xpt-pbuX, yqhZ-folD, and pbuO.
J Bacteriol: 2001, 183(21);6175-83
[PubMed:11591660] [WorldCat.org] [DOI] (P p)

D J Ebbole, H Zalkin
Interaction of a putative repressor protein with an extended control region of the Bacillus subtilis pur operon.
J Biol Chem: 1989, 264(6);3553-61
[PubMed:2536750] [WorldCat.org] (P p)