GuaA

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  • Description: GMP synthase (glutamine-hydrolysing)

Gene name guaA
Synonyms guaB
Essential no
Product GMP synthetase (glutamine-hydrolysing)
Function biosynthesis of GMP
Gene expression levels in SubtiExpress: guaA
Metabolic function and regulation of this protein in SubtiPathways:
guaA
MW, pI 57 kDa, 4.747
Gene length, protein length 1539 bp, 513 aa
Immediate neighbours yebA, pbuG
Sequences Protein DNA DNA_with_flanks
Genetic context
GuaA context.gif
This image was kindly provided by SubtiList
Expression at a glance   PubMed
GuaA expression.png















Categories containing this gene/protein

biosynthesis/ acquisition of nucleotides, most abundant proteins

This gene is a member of the following regulons

The gene

Basic information

  • Locus tag: BSU06360

Phenotypes of a mutant

Database entries

  • DBTBS entry: [1]
  • SubtiList entry: [2]

Additional information

The protein

Basic information/ Evolution

  • Catalyzed reaction/ biological activity: ATP + xanthosine 5'-phosphate + L-glutamine + H2O = AMP + diphosphate + GMP + L-glutamate (according to Swiss-Prot)
  • Protein family:
  • Paralogous protein(s):

Extended information on the protein

  • Kinetic information:
  • Modification:

Database entries

  • Structure: 2YWB (from Thermus thermophilus hb8, 45% identity, 57% similarity)
  • KEGG entry: [3]

Additional information

Expression and regulation

  • Regulation:
  • Regulatory mechanism:
  • Additional information:
    • belongs to the 100 most abundant proteins PubMed
    • number of protein molecules per cell (minimal medium with glucose and ammonium): 2612 PubMed
    • number of protein molecules per cell (complex medium with amino acids, without glucose): 12454 PubMed
    • number of protein molecules per cell (minimal medium with glucose and ammonium, exponential phase): 4578 PubMed
    • number of protein molecules per cell (minimal medium with glucose and ammonium, early stationary phase after glucose exhaustion): 5511 PubMed
    • number of protein molecules per cell (minimal medium with glucose and ammonium, late stationary phase after glucose exhaustion): 7521 PubMed

Biological materials

  • Mutant:
  • Expression vector:
  • lacZ fusion:
  • GFP fusion:
  • two-hybrid system:
  • Antibody:

Labs working on this gene/protein

Your additional remarks

References