Difference between revisions of "GuaA"

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=Expression and regulation=
 
=Expression and regulation=
  
* '''Operon:'''  
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* '''Operon:''' ''guaA'' {{PubMed|1312531}}
 
 
* '''[[Sigma factor]]:'''  
 
  
 +
* '''[[Sigma factor]]:''' [[SigA]] {{PubMed|1312531}}
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* '''Regulation:'''  
 
* '''Regulation:'''  
  
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=References=
 
=References=
  
<pubmed>1722815,, </pubmed>
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<pubmed>1722815,1312531, </pubmed>
  
 
[[Category:Protein-coding genes]]
 
[[Category:Protein-coding genes]]

Revision as of 19:20, 27 August 2009

  • Description: GMP synthase (glutamine-hydrolysing)

Gene name guaA
Synonyms guaB
Essential no
Product GMP synthetase (glutamine-hydrolysing)
Function biosynthesis of GMP
Metabolic function and regulation of this protein in SubtiPathways:
Purine synthesis, Nucleotides (regulation)
MW, pI 57 kDa, 4.747
Gene length, protein length 1539 bp, 513 aa
Immediate neighbours yebA, pbuG
Get the DNA and protein sequences
(Barbe et al., 2009)
Genetic context
GuaA context.gif
This image was kindly provided by SubtiList







The gene

Basic information

  • Locus tag: BSU06360

Phenotypes of a mutant

Database entries

  • DBTBS entry: [1]
  • SubtiList entry: [2]

Additional information

The protein

Basic information/ Evolution

  • Catalyzed reaction/ biological activity: ATP + xanthosine 5'-phosphate + L-glutamine + H2O = AMP + diphosphate + GMP + L-glutamate (according to Swiss-Prot)
  • Protein family:
  • Paralogous protein(s):

Extended information on the protein

  • Kinetic information:
  • Domains:
  • Modification:
  • Cofactor(s):
  • Effectors of protein activity:
  • Interactions:
  • Localization:

Database entries

  • Structure:
  • KEGG entry: [3]

Additional information

Expression and regulation

  • Regulation:
  • Regulatory mechanism:
  • Additional information:

Biological materials

  • Mutant:
  • Expression vector:
  • lacZ fusion:
  • GFP fusion:
  • two-hybrid system:
  • Antibody:

Labs working on this gene/protein

Your additional remarks

References

P Mäntsälä, H Zalkin
Cloning and sequence of Bacillus subtilis purA and guaA, involved in the conversion of IMP to AMP and GMP.
J Bacteriol: 1992, 174(6);1883-90
[PubMed:1312531] [WorldCat.org] [DOI] (P p)

H H Saxild, P Nygaard
Regulation of levels of purine biosynthetic enzymes in Bacillus subtilis: effects of changing purine nucleotide pools.
J Gen Microbiol: 1991, 137(10);2387-94
[PubMed:1722815] [WorldCat.org] [DOI] (P p)