GsiB

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  • Description: general stress protein, prevents enzyme inactivation upon freeze-thaw treatments

Gene name gsiB
Synonyms
Essential no
Product general stress protein
Function response to water deficits
Gene expression levels in SubtiExpress: gsiB
MW, pI 13 kDa, 5.145
Gene length, protein length 369 bp, 123 aa
Immediate neighbours ydbA, ydbB
Sequences Protein DNA DNA_with_flanks
Genetic context
GsiB context.gif
This image was kindly provided by SubtiList
[None Expression at a glance]   PubMed
GsiB expression.png















Categories containing this gene/protein

general stress proteins (controlled by SigB)

This gene is a member of the following regulons

SigB regulon, SigI regulon

The gene

Basic information

  • Locus tag: BSU04400

Phenotypes of a mutant

Database entries

  • DBTBS entry: [1]
  • SubtiList entry: [2]

Additional information

The protein

Basic information/ Evolution

  • Catalyzed reaction/ biological activity:
  • Protein family:
  • Paralogous protein(s):

Extended information on the protein

  • Kinetic information:
  • Domains:
  • Modification:
  • Cofactor(s):
  • Effectors of protein activity:

Database entries

  • Structure:
  • KEGG entry: [3]
  • E.C. number:

Additional information

Expression and regulation

  • Regulatory mechanism:
  • Additional information: the mRNA is very stable (half-life > 15 min) PubMed

Biological materials

  • Mutant:
  • Expression vector:
  • lacZ fusion:
  • GFP fusion:
  • two-hybrid system:
  • Antibody:

Labs working on this gene/protein

Your additional remarks

References

F Campos, C Cuevas-Velazquez, M A Fares, J L Reyes, A A Covarrubias
Group 1 LEA proteins, an ancestral plant protein group, are also present in other eukaryotes, and in the archeae and bacteria domains.
Mol Genet Genomics: 2013, 288(10);503-17
[PubMed:23861025] [WorldCat.org] [DOI] (I p)

Ioanna-Areti Asteri, Effrossyni Boutou, Rania Anastasiou, Bruno Pot, Constantinos E Vorgias, Effie Tsakalidou, Konstantinos Papadimitriou
In silico evidence for the horizontal transfer of gsiB, a σ(B)-regulated gene in gram-positive bacteria, to lactic acid bacteria.
Appl Environ Microbiol: 2011, 77(10);3526-31
[PubMed:21421783] [WorldCat.org] [DOI] (I p)

G Hambraeus, C von Wachenfeldt, L Hederstedt
Genome-wide survey of mRNA half-lives in Bacillus subtilis identifies extremely stable mRNAs.
Mol Genet Genomics: 2003, 269(5);706-14
[PubMed:12884008] [WorldCat.org] [DOI] (P p)

A Petersohn, M Brigulla, S Haas, J D Hoheisel, U Völker, M Hecker
Global analysis of the general stress response of Bacillus subtilis.
J Bacteriol: 2001, 183(19);5617-31
[PubMed:11544224] [WorldCat.org] [DOI] (P p)

B Jürgen, T Schweder, M Hecker
The stability of mRNA from the gsiB gene of Bacillus subtilis is dependent on the presence of a strong ribosome binding site.
Mol Gen Genet: 1998, 258(5);538-45
[PubMed:9669336] [WorldCat.org] [DOI] (P p)

B Maul, U Völker, S Riethdorf, S Engelmann, M Hecker
sigma B-dependent regulation of gsiB in response to multiple stimuli in Bacillus subtilis.
Mol Gen Genet: 1995, 248(1);114-20
[PubMed:7651322] [WorldCat.org] [DOI] (P p)

J P Mueller, G Bukusoglu, A L Sonenshein
Transcriptional regulation of Bacillus subtilis glucose starvation-inducible genes: control of gsiA by the ComP-ComA signal transduction system.
J Bacteriol: 1992, 174(13);4361-73
[PubMed:1378051] [WorldCat.org] [DOI] (P p)