GroEL

• Description: chaperonin and co-repressor for HrcA
  
  

Gene name	groEL
Synonyms
Essential	yes PubMed
Product	chaperonin, co-repressor for HrcA
Function	protein folding and re-folding
Metabolic function and regulation of this protein in SubtiPathways: Stress
MW, pI	57 kDa, 4.531
Gene length, protein length	1632 bp, 544 aa
Immediate neighbours	groES, ydiM
Get the DNA and protein sequences (Barbe et al., 2009)
Genetic context This image was kindly provided by SubtiList

The gene

Basic information

• Locus tag: BSU06030

Phenotypes of a mutant

essential PubMed

Database entries

• DBTBS entry: [1]

• SubtiList entry: [2]

The protein

Basic information/ Evolution

• Catalyzed reaction/ biological activity: acts as co-repressor for HrcA

• Protein family: chaperonin (HSP60) family (according to Swiss-Prot)

• Paralogous protein(s):

Extended information on the protein

• Kinetic information:

• Domains:

• Modification: phosphorylated on ser/ thr/ tyr PubMed, PubMed

• Cofactor(s):

• Effectors of protein activity:

• Interactions: HrcA-GroEL, GroEL-GroES

• Localization: cytoplasm (according to Swiss-Prot)

Database entries

• Structure:

• UniProt: P28598

• KEGG entry: [3]

• E.C. number:

Expression and regulation

• Operon: groES-groEL PubMed

• Sigma factor: SigA PubMed

• Regulation: induced by heat shock (HrcA) PubMed

• Regulatory mechanism: HrcA: transcription repression PubMed

• Additional information:

Biological materials

• Mutant:

• Expression vector:

• lacZ fusion:

• GFP fusion:

• two-hybrid system:

• Antibody:

Labs working on this gene/protein

Wolfgang Schumann, Bayreuth University, Germany Homepage

References

Christine Eymann, Dörte Becher, Jörg Bernhardt, Katrin Gronau, Anja Klutzny, Michael Hecker
Dynamics of protein phosphorylation on Ser/Thr/Tyr in Bacillus subtilis.
Proteomics: 2007, 7(19);3509-26
[PubMed:17726680] [WorldCat.org] [DOI] (P p)

Alain Lévine, Françoise Vannier, Cédric Absalon, Lauriane Kuhn, Peter Jackson, Elaine Scrivener, Valérie Labas, Joëlle Vinh, Patrick Courtney, Jérôme Garin, Simone J Séror
Analysis of the dynamic Bacillus subtilis Ser/Thr/Tyr phosphoproteome implicated in a wide variety of cellular processes.
Proteomics: 2006, 6(7);2157-73
[PubMed:16493705] [WorldCat.org] [DOI] (P p)

Silke Reischl, Thomas Wiegert, Wolfgang Schumann
Isolation and analysis of mutant alleles of the Bacillus subtilis HrcA repressor with reduced dependency on GroE function.
J Biol Chem: 2002, 277(36);32659-67
[PubMed:12082092] [WorldCat.org] [DOI] (P p)

A Mogk, G Homuth, C Scholz, L Kim, F X Schmid, W Schumann
The GroE chaperonin machine is a major modulator of the CIRCE heat shock regulon of Bacillus subtilis.
EMBO J: 1997, 16(15);4579-90
[PubMed:9303302] [WorldCat.org] [DOI] (P p)

A Schulz, W Schumann
hrcA, the first gene of the Bacillus subtilis dnaK operon encodes a negative regulator of class I heat shock genes.
J Bacteriol: 1996, 178(4);1088-93
[PubMed:8576042] [WorldCat.org] [DOI] (P p)

A Schmidt, M Schiesswohl, U Völker, M Hecker, W Schumann
Cloning, sequencing, mapping, and transcriptional analysis of the groESL operon from Bacillus subtilis.
J Bacteriol: 1992, 174(12);3993-9
[PubMed:1350777] [WorldCat.org] [DOI] (P p)

M Li, S L Wong
Cloning and characterization of the groESL operon from Bacillus subtilis.
J Bacteriol: 1992, 174(12);3981-92
[PubMed:1350776] [WorldCat.org] [DOI] (P p)