GpsA

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  • Description: glycerol-3-phosphate dehydrogenase (NAD)

Gene name gpsA
Synonyms
Essential yes PubMed
Product glycerol-3-phosphate dehydrogenase (NAD)
Function biosynthesis of phospholipids
Gene expression levels in SubtiExpress: gpsA
Metabolic function and regulation of this protein in SubtiPathways:
gpsA
MW, pI 37 kDa, 6.231
Gene length, protein length 1035 bp, 345 aa
Immediate neighbours yphE, engA
Sequences Protein DNA DNA_with_flanks
Genetic context
GpsA context.gif
This image was kindly provided by SubtiList
Expression at a glance   PubMed
GpsA expression.png
















Categories containing this gene/protein

biosynthesis of lipids, essential genes,

This gene is a member of the following regulons

The gene

Basic information

  • Locus tag: BSU22830

Phenotypes of a mutant

essential PubMed

Database entries

  • DBTBS entry: no entry
  • SubtiList entry: [1]

Additional information

The protein

Basic information/ Evolution

  • Catalyzed reaction/ biological activity: sn-glycerol 3-phosphate + NAD(P)+ = glycerone phosphate + NAD(P)H (according to Swiss-Prot)
  • Protein family: NAD-dependent glycerol-3-phosphate dehydrogenase family (according to Swiss-Prot)
  • Paralogous protein(s):

Extended information on the protein

  • Kinetic information:
  • Domains:
  • Modification:
  • Cofactor(s):
  • Effectors of protein activity:

Database entries

  • Structure: 3K96 (from Coxiella burnetii RSA 493, 37% identity, 59% similarity)
  • KEGG entry: [2]

Additional information

Expression and regulation

  • Operon:
  • Sigma factor:
  • Regulation:
  • Regulatory mechanism:
  • Additional information:
    • number of protein molecules per cell (minimal medium with glucose and ammonium): 165 PubMed
    • number of protein molecules per cell (complex medium with amino acids, without glucose): 809 PubMed

Biological materials

  • Mutant:
  • Expression vector:
  • lacZ fusion:
  • GFP fusion:
  • two-hybrid system:
  • Antibody:

Labs working on this gene/protein

Your additional remarks

References

Ayako Nishibori, Jin Kusaka, Hiroshi Hara, Masato Umeda, Kouji Matsumoto
Phosphatidylethanolamine domains and localization of phospholipid synthases in Bacillus subtilis membranes.
J Bacteriol: 2005, 187(6);2163-74
[PubMed:15743965] [WorldCat.org] [DOI] (P p)

H R Morbidoni, D de Mendoza, J E Cronan
Synthesis of sn-glycerol 3-phosphate, a key precursor of membrane lipids, in Bacillus subtilis.
J Bacteriol: 1995, 177(20);5899-905
[PubMed:7592341] [WorldCat.org] [DOI] (P p)