GpsA

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  • Description: glycerol-3-phosphate dehydrogenase (NAD)

Gene name gpsA
Synonyms
Essential yes PubMed
Product glycerol-3-phosphate dehydrogenase (NAD)
Function biosynthesis of phospholipids
Metabolic function and regulation of this protein in SubtiPathways:
Lipid synthesis
MW, pI 37 kDa, 6.231
Gene length, protein length 1035 bp, 345 aa
Immediate neighbours yphE, yphC
Get the DNA and protein sequences
(Barbe et al., 2009)
Genetic context
GpsA context.gif
This image was kindly provided by SubtiList







Categories containing this gene/protein

biosynthesis of lipids, essential genes, membrane proteins

This gene is a member of the following regulons

The gene

Basic information

  • Locus tag: BSU22830

Phenotypes of a mutant

essential PubMed

Database entries

  • DBTBS entry: no entry
  • SubtiList entry: [1]

Additional information

The protein

Basic information/ Evolution

  • Catalyzed reaction/ biological activity: sn-glycerol 3-phosphate + NAD(P)+ = glycerone phosphate + NAD(P)H (according to Swiss-Prot)
  • Protein family: NAD-dependent glycerol-3-phosphate dehydrogenase family (according to Swiss-Prot)
  • Paralogous protein(s):

Extended information on the protein

  • Kinetic information:
  • Domains:
  • Modification:
  • Cofactor(s):
  • Effectors of protein activity:
  • Interactions:
  • Localization: cytoplasm PubMed

Database entries

  • Structure: 3K96 (from Coxiella burnetii RSA 493, 37% identity, 59% similarity)
  • KEGG entry: [2]

Additional information

Expression and regulation

  • Operon:
  • Regulation:
  • Regulatory mechanism:
  • Additional information:

Biological materials

  • Mutant:
  • Expression vector:
  • lacZ fusion:
  • GFP fusion:
  • two-hybrid system:
  • Antibody:

Labs working on this gene/protein

Your additional remarks

References

Ayako Nishibori, Jin Kusaka, Hiroshi Hara, Masato Umeda, Kouji Matsumoto
Phosphatidylethanolamine domains and localization of phospholipid synthases in Bacillus subtilis membranes.
J Bacteriol: 2005, 187(6);2163-74
[PubMed:15743965] [WorldCat.org] [DOI] (P p)

H R Morbidoni, D de Mendoza, J E Cronan
Synthesis of sn-glycerol 3-phosphate, a key precursor of membrane lipids, in Bacillus subtilis.
J Bacteriol: 1995, 177(20);5899-905
[PubMed:7592341] [WorldCat.org] [DOI] (P p)