Difference between revisions of "GndA"

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(Additional information)
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=== Additional information===
 
=== Additional information===
It contains a cystein on the active site. The enzyme is a dimer. [http://www.ncbi.nlm.nih.gov/sites/entrez/15231785 PubMed]
+
It contains a cysteine on the active site. The enzyme is a dimer. [http://www.ncbi.nlm.nih.gov/sites/entrez/15231785 PubMed]
  
 
=Expression and regulation=
 
=Expression and regulation=

Revision as of 20:39, 18 November 2009

  • Description: NADP-dependent phosphogluconate dehydrogenase

Gene name gndA
Synonyms yqjI
Essential no
Product NADP-dependent phosphogluconate dehydrogenase
Function pentose phosphate pathway
Metabolic function and regulation of this protein in SubtiPathways:
Central C-metabolism
MW, pI 51 kDa, 5.076
Gene length, protein length 1407 bp, 469 aa
Immediate neighbours zwf, polY1
Get the DNA and protein sequences
(Barbe et al., 2009)
Genetic context
YqjI context.gif
This image was kindly provided by SubtiList







The gene

Basic information

  • Locus tag: BSU23860

Phenotypes of a mutant

Database entries

  • DBTBS entry: [1]
  • SubtiList entry: [2]

Additional information

The protein

Basic information/ Evolution

  • Catalyzed reaction/ biological activity: 6-phospho-D-gluconate + NADP+ = D-ribulose 5-phosphate + CO2 + NADPH (according to Swiss-Prot)
  • Protein family: 6-phosphogluconate dehydrogenase family (according to Swiss-Prot)
  • Paralogous protein(s):

Extended information on the protein

  • Kinetic information: Reversible reaction PubMed
  • Domains:
  • Modification: phosphorylation on (Thr-15 OR Thr-17) PubMed
  • Cofactor(s):
  • Effectors of protein activity:
  • Interactions:
  • Localization:

Database entries

  • Structure:
  • KEGG entry: [3]

Additional information

It contains a cysteine on the active site. The enzyme is a dimer. PubMed

Expression and regulation

  • Operon:
  • Regulation:
  • Regulatory mechanism:
  • Additional information:

Biological materials

  • Mutant:
  • Expression vector:
  • lacZ fusion:
  • GFP fusion:
  • two-hybrid system:
  • Antibody:

Labs working on this gene/protein

Your additional remarks

References

Boris Macek, Ivan Mijakovic, Jesper V Olsen, Florian Gnad, Chanchal Kumar, Peter R Jensen, Matthias Mann
The serine/threonine/tyrosine phosphoproteome of the model bacterium Bacillus subtilis.
Mol Cell Proteomics: 2007, 6(4);697-707
[PubMed:17218307] [WorldCat.org] [DOI] (P p)

Nicola Zamboni, Eliane Fischer, Dietmar Laudert, Stéphane Aymerich, Hans-Peter Hohmann, Uwe Sauer
The Bacillus subtilis yqjI gene encodes the NADP+-dependent 6-P-gluconate dehydrogenase in the pentose phosphate pathway.
J Bacteriol: 2004, 186(14);4528-34
[PubMed:15231785] [WorldCat.org] [DOI] (P p)