gmuG

168

beta-1,4-mannanase

locus: BSU_05880

Molecular weight: 40.68 kDa

pI: 5.63

Protein length: 362 aa

Gene length: 1089 bp

function: glucomannan utilization

product: beta-1,4-mannanase

essential: no

ec: 3.2.1.78

synonyms: gmuG, ydhT

List of homologs in different organisms, belongs to COG4124 (Galperin et al., 2021)

Gene

Coordinates: 632,774  633,862

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The protein

Catalyzed reaction/ biological activity

• Random hydrolysis of (14)-beta-D-mannosidic linkages in mannans, galactomannans and glucomannans (according to UniProt)

Protein family

• glycosyl hydrolase 26 family (single member, according to UniProt)

[wiki|Domains]

• GH26 domain (aa 38-349) (according to UniProt)

Structure

• [PDB|2QHA], [http://www.proteopedia.org/wiki/index.php/3cbw 3CBW] mutant variant
• [AF|O05512]

[wiki|Localization]

• extracellular (signal peptide) [Pubmed|18957862]

Biological materials

Mutant

• MGNA-C195 (ydhT::erm), available at the [https://shigen.nig.ac.jp/bsub/resource/strainGeneDisrupted/detail/2193 NBRP B. subtilis, Japan]
• BKE05880 ([gene|289ACC9D13BBFAA5C083EE525ECCC32DB06E075F|gmuG]::erm  trpC2) available at [http://bgsc.org/getdetail.php?bgscid=BKE05880 BGSC],  [Pubmed|28189581], upstream reverse: _UP1_CAAATGCAACTCCCCCATTC,  downstream forward: _UP4_TGAATCCGTAATGTCACAAT
• BKK05880 ([gene|289ACC9D13BBFAA5C083EE525ECCC32DB06E075F|gmuG]::kan  trpC2) available at [http://bgsc.org/getdetail.php?bgscid=BKK05880 BGSC],  [Pubmed|28189581], upstream reverse: _UP1_CAAATGCAACTCCCCCATTC,  downstream forward: _UP4_TGAATCCGTAATGTCACAAT

References

• 19504356,18177310,18957862,19441796,20709850,20817675,24421132