gmuF

168

mannose-6-P-isomerase

locus: BSU_05870

Molecular weight: 35.34 kDa

pI: 5.89

Protein length: 315 aa

Gene length: 948 bp

function: glucomannan utilization

product: mannose-6-P-isomerase

essential: no

synonyms: gmuF, ydhS

List of homologs in different organisms, belongs to COG1482 (Galperin et al., 2021)

Gene

Coordinates: 631,808  632,755

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The protein

Catalyzed reaction/ biological activity

• D-mannose 6-phosphate --> D-fructose 6-phosphate (according to UniProt)

Protein family

• mannose-6-phosphate isomerase type 1 family (with [protein|E26C70893C5D677C816C814558CC42F90B920087|manA] and [protein|AC79E2A80664B4D165A0AE46CEA124C57EB9770D|pmi], according to UniProt)

Structure

• [PDB|1QWR] ([protein|AC79E2A80664B4D165A0AE46CEA124C57EB9770D|pmi], 58% identity)
• [AF|O05511]

Paralogous protein(s)

• [protein|AC79E2A80664B4D165A0AE46CEA124C57EB9770D|pmi], [protein|E26C70893C5D677C816C814558CC42F90B920087|manA]

Biological materials

Mutant

• MGNA-C194 (ydhS::erm), available at the [https://shigen.nig.ac.jp/bsub/resource/strainGeneDisrupted/detail/2192 NBRP B. subtilis, Japan]
• BKE05870 ([gene|35225776F6044513D3E77DBBD6A7A8FE976F506A|gmuF]::erm  trpC2) available at [http://bgsc.org/getdetail.php?bgscid=BKE05870 BGSC],  [Pubmed|28189581], upstream reverse: _UP1_AGGCTCTAAAAATAATGGAT,  downstream forward: _UP4_CCTTAATGAATGGGGGAGTT
• BKK05870 ([gene|35225776F6044513D3E77DBBD6A7A8FE976F506A|gmuF]::kan  trpC2) available at [http://bgsc.org/getdetail.php?bgscid=BKK05870 BGSC],  [Pubmed|28189581], upstream reverse: _UP1_AGGCTCTAAAAATAATGGAT,  downstream forward: _UP4_CCTTAATGAATGGGGGAGTT

References

• 18177310,19447949,20817675